ArticleToxicology and applied pharmacology2012
Protective role for ovarian glutathione S-transferase isoform pi during 7,12-dimethylbenz[a]anthracene-induced ovotoxicity.
Article in Toxicology and applied pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Prenatal and postnatal atrazine-induced endocrine and ovarian chemical biotransformation protein disruption in offspring.Journal of the Endocrine Society · 2026Article
- Investigating ovarian effects of perfluorooctanoic acid in postpubertal pigs during thermal neutral and heat stress conditions.Journal of animal science · 2026Article
- Diet-induced obesity alters the ovarian chemical biotransformation and oxidative stress response proteins both basally and in response to 7,12-dimethylbenz[a]anthracene exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- High fat diet-induced obesity and gestational DMBA exposure alter folliculogenesis and the proteome of the maternal ovary†.Biology of reproduction · 2024Article
- Zearalenone exposure differentially affects the ovarian proteome in pre-pubertal gilts during thermal neutral and heat stress conditions.Journal of animal science · 2024Article
- Maternal pre-conceptional glyphosate exposure impacts the offspring hepatic and ovarian proteome.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Ovarian mitochondrial and oxidative stress proteins are altered by glyphosate exposure in mice.Toxicology and applied pharmacology · 2020Article
- Involvement of a volatile metabolite during phosphoramide mustard-induced ovotoxicity.Toxicology and applied pharmacology · 2014Article
- Acute 7,12-dimethylbenz[a]anthracene exposure causes differential concentration-dependent follicle depletion and gene expression in neonatal rat ovaries.Toxicology and applied pharmacology · 2014Article
- Impact of obesity on ovotoxicity induced by 7,12-dimethylbenz[a]anthracene in mice.Biology of reproduction · 2014Article
- High fat diet induced obesity alters ovarian phosphatidylinositol-3 kinase signaling gene expression.Reproductive toxicology (Elmsford, N.Y.) · 2013Article
- Glutathione S-transferase class μ regulation of apoptosis signal-regulating kinase 1 protein during VCD-induced ovotoxicity in neonatal rat ovaries.Toxicology and applied pharmacology · 2013Article
- Article
- Review
- Impact of environmental exposures on ovarian function and role of xenobiotic metabolism during ovotoxicity.Toxicology and applied pharmacology · 2012Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
7,12-Dimethylbenz[a]anthracene (DMBA) destroys ovarian follicles at all developmental stages. This study investigated a role for the glutathione S-transferase (Gst) isoforms alpha (a), mu (m) and pi (p) and the transcription factors, Ahr and Nrf2, during DMBA-induced ovotoxicity, and their regulation by phosphatidylinositol-3 kinase (PI3K) signaling. Negative regulation of JNK by GSTP during DMBA exposure was also studied. Post-natal day (PND) 4 Fischer 344 rat ovaries were exposed to vehicle control (1% DMSO)±DMBA (1 μM) or vehicle control (1% DMSO)±LY294002 (PI3K inhibitor; 20 μM) for 1, 2, 4, or 6 days. Total RNA or protein was isolated, followed by RT-PCR or Western blotting to determine mRNA or protein level, respectively. Immunoprecipitation using an anti-GSTP antibody was performed to determine interaction between GSTP and JNK, followed by Western blotting to determine JNK and p-c-Jun protein level. DMBA had no impact on Gsta, Gstm or Nrf2 mRNA level, but increased Gstp mRNA and protein after 2 days. Ahr mRNA and protein increased after 2 and 4 days of DMBA exposure, respectively and DMBA increased NRF2 protein level after 4 days. JNK bound to GSTP was increased during DMBA exposure, with a concomitant decrease in unbound JNK and p-c-Jun. Ahr and Gstp mRNA were decreased (2 days) and increased (4 days) by PI3K inhibition, while Gstm mRNA increased (P<0.05) after both time points, and there was no effect on Nrf2 mRNA. PI3K inhibition increased AHR, NRF2 and GSTP protein level. These findings support involvement of ovarian GSTP during DMBA exposure, and indicate a regulatory role for the PI3K signaling pathway on ovarian xenobiotic metabolism gene expression.
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