ArticleInsect biochemistry and molecular biology2012
Identification and developmental profiling of conserved and novel microRNAs in Manduca sexta.
Article in Insect biochemistry and molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 69 citations in OpenAlex.
- Repeated emergence of giant microRNA hairpins across invertebrates.Cell reports · 2025Article
- Non-coding RNAs identification and regulatory networks in pathogen-host interaction in the microsporidia congenital infection.BMC genomics · 2023Article
- High Daytime Temperature Responsive MicroRNA Profiles in Developing Grains of Rice Varieties with Contrasting Chalkiness.International journal of molecular sciences · 2023Article
- A Genetic Compensation Phenomenon and Global Gene Expression Changes in Sex-miR-2766-3p Knockout Strain ofInsects · 2022Article
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- microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing.PloS one · 2022Article
- miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta.Scientific reports · 2021Article
- High-resolution analysis of differential gene expression during skeletal muscle atrophy and programmed cell death.Physiological genomics · 2020Article
- Post-transcriptional regulation of insect metamorphosis and oogenesis.Cellular and molecular life sciences : CMLS · 2020Review
- Identification and Integrated Analysis of MicroRNA and mRNA Expression Profiles During Agonistic Behavior in Chinese Mitten Crab (Frontiers in genetics · 2020Article
- The Role of MicroRNAs in Genome Response to Plant-Lepidoptera Interaction.Plants (Basel, Switzerland) · 2019Review
- Genome-wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips,Ecology and evolution · 2018Article
- Genome-Wide Identification of Destruxin A-Responsive Immunity-Related MicroRNAs in Diamondback Moth,Frontiers in immunology · 2018Article
- An analysis of 67 RNA-seq datasets from various tissues at different stages of a model insect, Manduca sexta.BMC genomics · 2017Article
- Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types.BMC genomics · 2017Article
- Identification of microRNAs and their response to the stress of plant allelochemicals in Aphis gossypii (Hemiptera: Aphididae).BMC molecular biology · 2017Article
- MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.Scientific reports · 2017Article
- Article
- Genome-Wide Profiling ofFrontiers in physiology · 2017Article
- Comparative profiling of microRNAs in the winged and wingless English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae).Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
MicroRNAs (miRNAs) are a group of small RNAs involved in translation inhibition or mRNA degradation. Due to its large size, Manduca sexta has long been used as a model to study insect physiology and biochemistry. While transcriptome studies have greatly enriched our knowledge on M. sexta structural genes, little is known about posttranscriptional regulation by miRNAs in this lepidopteran species. We constructed four small RNA libraries from embryos, 4th instar feeding larvae, pupae, and adults, obtained 21 million reads of 18-31 nucleotides by Illumina sequencing, and found 163 conserved and 13 novel miRNAs. By searching the M. sexta genome assembly, we identified precursors of 82 conserved miRNAs, 76 of which had mapped reads in one or more of these libraries. After normalization, we compared numbers of miRNA and miRNA-star reads in these libraries and observed abundance changes during development. Interestingly, mse-miR-281-star, mse-miR-31-star, mse-miR-965-star, mse-miR-9a-star, mse-miR-9b-star, mse-miR-2a-star, mse-miR-92b-star and mse-miR-279c-star are either more abundant or maintained at similar levels compared to respective mature miRNA strand. Expression profiling of the first set of miRNAs provided insights to their possible involvement in developmental regulation. This study will aid in the annotation of miRNA genes in the genome.
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Registered trials
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