ArticleBiophysical journal2012
In vivo imaging of the actin polymerization state with two-photon fluorescence anisotropy.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
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- Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association.Nature communications · 2020Article
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- A concept for single-shot volumetric fluorescence imaging via orthogonally polarized excitation lattices.Scientific reports · 2019Article
- Using enhanced number and brightness to measure protein oligomerization dynamics in live cells.Nature protocols · 2019Article
- Homotransfer of FRET Reporters for Live Cell Imaging.Biosensors · 2018Review
- Characterization of Split Fluorescent Protein Variants and Quantitative Analyses of Their Self-Assembly Process.Scientific reports · 2018Article
- Total internal reflection fluorescence anisotropy imaging microscopy: setup, calibration, and data processing for protein polymerization measurements in living cells.Methods and applications in fluorescence · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Super-resolution dipole orientation mapping via polarization demodulation.Light, science & applications · 2016Article
- Role of intracellular poroelasticity on freezing-induced deformation of cells in engineered tissues.Journal of the Royal Society, Interface · 2016Article
- Quantitative nanoscale imaging of orientational order in biological filaments by polarized superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- 3D fluorescence anisotropy imaging using selective plane illumination microscopy.Optics express · 2015Article
- Fluorescence anisotropy (polarization): from drug screening to precision medicine.Expert opinion on drug discovery · 2015Review
- In vivo imaging of specific drug-target binding at subcellular resolution.Nature communications · 2014Article
- Measuring F-actin properties in dendritic spines.Frontiers in neuroanatomy · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Using two-photon fluorescence anisotropy imaging of actin-GFP, we have developed a method for imaging the actin polymerization state that is applicable to a broad range of experimental systems extending from fixed cells to live animals. The incorporation of expressed actin-GFP monomers into endogenous actin polymers enables energy migration FRET (emFRET, or homoFRET) between neighboring actin-GFPs. This energy migration reduces the normally high polarization of the GFP fluorescence. We derive a simple relationship between the actin-GFP fluorescence polarization anisotropy and the actin polymer fraction, thereby enabling a robust means of imaging the actin polymerization state with high spatiotemporal resolution and providing what to the best of our knowledge are the first direct images of the actin polymerization state in live, adult brain tissue and live, intact Drosophila larvae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.