ArticleBiophysical journal2012
Superoxide flashes reveal novel properties of mitochondrial reactive oxygen species excitability in cardiomyocytes.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- The Switching of the Type of a ROS Signal from Mitochondria: The Role of Respiratory Substrates and Permeability Transition.Antioxidants (Basel, Switzerland) · 2024Article
- BacFlash signals acid-resistance gene expression in bacteria.Cell research · 2021Article
- What NIR photodynamic activation offers molecular targeted nanomedicines: Perspectives into the conundrum of tumor specificity and selectivity.Nano today · 2021Article
- Dual Targeting of Endothelial and Cancer Cells Potentiates In Vitro Nanobody-Targeted Photodynamic Therapy.Cancers · 2020Article
- Protocol for Imaging of Mitoflashes in Live Cardiomyocytes.STAR protocols · 2020Article
- A 20/20 view of ANT function in mitochondrial biology and necrotic cell death.Journal of molecular and cellular cardiology · 2020Review
- Integrating Ultra-Weak Photon Emission Analysis in Mitochondrial Research.Frontiers in physiology · 2020Review
- The Role of Mitochondria in the Mechanisms of Cardiac Ischemia-Reperfusion Injury.Antioxidants (Basel, Switzerland) · 2019Review
- Mitoflash biogenesis and its role in the autoregulation of mitochondrial proton electrochemical potential.The Journal of general physiology · 2019Article
- Isolating a reverse-mode ATP synthase-dependent mechanism of mitoflash activation.The Journal of general physiology · 2019Article
- Article
- ROS-related mitochondrial dysfunction in skeletal muscle of an ALS mouse model during the disease progression.Pharmacological research · 2018Article
- Ultrastructural Characterization of Flashing Mitochondria.Contact (Thousand Oaks (Ventura County, Calif.)) · 2018Article
- Reactive Oxygen Species as Intracellular Signaling Molecules in the Cardiovascular System.Current cardiology reviews · 2018Review
- Mitochondrial permeability transition in cardiac ischemia-reperfusion: whether cyclophilin D is a viable target for cardioprotection?Cellular and molecular life sciences : CMLS · 2017Review
- Article
- Absence of physiological CaSkeletal muscle · 2017Article
- Mitochondrial flashes: From indicator characterization to in vivo imaging.Methods (San Diego, Calif.) · 2016Article
- Mitochondrial Flash: Integrative Reactive Oxygen Species and pH Signals in Cell and Organelle Biology.Antioxidants & redox signaling · 2016Review
- Regulation of Mitoflash Biogenesis and Signaling by Mitochondrial Dynamics.Scientific reports · 2016Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superoxide flash represents quantal and bursting production of mitochondrial reactive oxygen species (ROS) instigated by transient opening of the mitochondrial permeability transition pore (mPTP). Given their critical role in metabolism, ischemia-reperfusion injury, and apoptosis, characterization of flash properties would be valuable to further mechanistic and physiological studies of this newly discovered mitochondrial phenomenon. Here we developed the flash detector FlashSniper based on segmentation of two-dimensional feature maps extracted from time-lapse confocal image stacks, and on the theory for correcting optical distortion of flash-amplitude histograms. Through large-scale analysis of superoxide flashes in cardiomyocytes, we demonstrated uniform mitochondrial ROS excitability among subsarcolemmal and intermyofibrillar mitochondria, and exponential distribution of intervals between consecutive flash events. Flash ignition displayed three different patterns: an abrupt rise from quiescence (44%), a rise with an exponential foot (27%), or a rise occurring after a pedestal precursor (29%), closely resembling action-potential initiation in excitable cells. However, the optical blurring-corrected amplitudes of superoxide flashes were highly variable, as were their durations, indicating stochastic automaticity of single-mitochondrion ROS excitation. Simultaneous measurement of mitochondrial membrane potential revealed that graded, rather than all-or-none, depolarization mirrored the precursor and the primary peak of the flash. We propose that superoxide flash production is a regenerative process dominated by stochastic, autonomous recruitment of a limited number of units (e.g., mPTPs) in single mitochondria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.