ArticlePlant methods2012
A novel procedure for absolute real-time quantification of gene expression patterns.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- Recognition of a Fungal Effector Potentiates Pathogen-Associated Molecular Pattern-Triggered Immunity in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A Regulatory Mechanism on Pathways: Modulating Roles of MYC2 and BBX21 in the Flavonoid Network.Plants (Basel, Switzerland) · 2024Article
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- Characterization of Cytochrome P450s with Key Roles in Determining Herbicide Selectivity in Maize.ACS omega · 2022Article
- Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis.Scientific reports · 2021Observational
- Cardiac‑specific deletion of natriuretic peptide receptor A induces differential myocardial expression of circular RNA and mRNA molecules involved in metabolism in mice.Molecular medicine reports · 2021Article
- Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples.PloS one · 2021Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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- Molecular characterization of alterations in the intestinal microbiota of patients with grade 3 hypertension.International journal of molecular medicine · 2019Article
- A Genome-Wide Association Study Identifies New Loci Involved in Wound-Induced Lateral Root Formation inFrontiers in plant science · 2019Article
- Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings.Frontiers in plant science · 2018Article
- Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina.Scientific reports · 2017Article
- Absolute quantification of cassava brown streak virus mRNA by real-time qPCR.Journal of virological methods · 2017Article
- Evolution of Chaperonin Gene Duplication in Stigonematalean Cyanobacteria (Subsection V).Genome biology and evolution · 2017Article
- Article
- Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.Journal of experimental botany · 2015Article
- Identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (Zea mays L.) under abiotic and biotic stresses.Frontiers in plant science · 2015Article
- Biochemical and molecular characterization of RcSUS1, a cytosolic sucrose synthase phosphorylated in vivo at serine 11 in developing castor oil seeds.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTemporal and tissue-specific patterns of gene expression play important roles in functionality of a biological system. Real-time quantitative polymerase chain reaction (qPCR) technique has been widely applied to single gene expressions, but its potential has not been fully released as most results have been obtained as fold changes relative to control conditions. Absolute quantification of transcripts as an alternative method has yet to gain popularity because of unresolved issues.
resultsWe propose a solution here with a novel procedure, which may accurately quantify the total cDNA conventionally prepared from a biological sample at the resolution of ~70 pg/μl, and reliably estimate the absolute numbers of transcripts in a picogram of cDNA. In comparison to the relative quantification, cDNA-based absolute (CBA) qPCR method is found to be more sensitive to gene expression variations caused by factors such as developmental and environmental variations. If the number of target transcript copies is further normalized by reference transcripts, cell-level variation pattern of the target gene expression may also be detectable during a developmental process, as observed here in cases across species (Ipomoea purpurea, Nicotiana benthamiana) and tissues (petals and leaves).
conclusionBy allowing direct comparisons of results across experiments, the new procedure opens a window to make inferences of gene expression patterns across a broad spectrum of living systems and tissues. Such comparisons are urgently needed for biological interpretations of gene expression variations in diverse cells.
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Registered trials
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