ArticleHelicobacter2012
Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children.
Article in Helicobacter, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Infiltration to infection: key virulence players of Helicobacter pylori pathogenicity.Infection · 2024Pooled it
- Molecular Detection and Clinical Impact ofBiomedicines · 2025Article
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- Reactive Oxygen Species andAntioxidants (Basel, Switzerland) · 2023Review
- The Importance of Accurate Early Diagnosis and Eradication inAntibiotics (Basel, Switzerland) · 2022Review
- ZAK Gene Expression in Patients with Helicobacter pylori Infection.Journal of gastrointestinal cancer · 2022Article
- Sirt3, 6, and 7 Genes Expression in Gastric Antral Epithelial Cells of Patients with Helicobacter pylori Infection.Current microbiology · 2022Article
- DNMT1 Gene Expression in Patients withTheScientificWorldJournal · 2022Article
- Review
- Article
- Methods for detection of Helicobacter pylori from stool sample: current options and developments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Transcriptional Profile ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021Article
- Review
- Effects of Concurrent Omega-3 and Cranberry Juice Consumption Along with Standard Antibiotic Therapy on the Eradication ofInfection and drug resistance · 2020Article
- Review
- Helicobacter pylori infection in children: an overview of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomaticArchives of medical science : AMS · 2019Article
- Article
- Article
- Diagnosis of Helicobacter pylori infection: Current options and developments.World journal of gastroenterology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundHelicobacter pylori infection is usually acquired in childhood, but little is known about its natural history in asymptomatic children, primarily due to the paucity of non-invasive diagnostic methods. H. pylori strains harboring cagA and specific alleles of hopQ and vacA are associated with increased risk for gastric cancer. Many studies of H. pylori virulence markers in children have the bias that symptomatic subjects are selected for endoscopy, and these children may harbor the most virulent strains. Our aim is to genotype cagA, hopQ, and vacA alleles in stool DNA samples of healthy Colombian children residing in an area with high incidence of gastric cancer, to avoid selection bias resulting from endoscopy.
methodsH. pylori status of 86 asymptomatic children was assessed by (13) C-urea breath test (UBT) and PCR. H. pylori 16S rRNA, cagA, hopQ, and vacA genes were amplified from stool DNA samples and sequenced.
resultsUBT was positive in 69 (80.2%) of 86 children; in stool DNA analysis, 78.3% were positive by 16S rRNA PCR. cagA, vacA, and hopQ were detected in 66.1%, 84.6%, and 72.3% of stool DNA samples from 16S rRNA-positive children. Of the children's DNA samples, which revealed vacA and hopQ alleles, 91.7% showed vacA s1 and 73.7% showed type I hopQ. Type I hopQ alleles were associated with cagA positivity and vacA s1 genotypes (p < 0.0001).
conclusionsUsing stool DNA samples, virulence markers of H. pylori were successfully genotyped in a high percentage of the asymptomatic infected children, revealing a high prevalence of genotypes associated with virulence. Type I hopQ alleles were associated with the presence of cagA and the vacA s1 genotype.
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