ArticleAmerican journal of physiology. Cell physiology2012
Functional Cftr in crypt epithelium of organotypic enteroid cultures from murine small intestine.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- Functional maturation of preterm intestinal epithelium through CFTR activation.Communications biology · 2025Article
- A Decade of Organoid Research: Progress and Challenges in the Field of Organoid Technology.ACS omega · 2024Review
- Intracellular pH dynamics regulates intestinal stem cell lineage specification.Nature communications · 2023Article
- Restoration of the gut barrier integrity and restructuring of the gut microbiome in aging by angiotensin-(1-7).Clinical science (London, England : 1979) · 2023Article
- Crosslink between SARS-CoV-2 replication and cystic fibrosis hallmarks.Frontiers in microbiology · 2023Review
- Stem Cell Therapy in Inflammatory Bowel Disease: A Review of Achievements and Challenges.Journal of inflammation research · 2023Review
- The NHE3 Inhibitor Tenapanor Prevents Intestinal Obstructions in CFTR-Deleted Mice.International journal of molecular sciences · 2022Article
- CFTR and Gastrointestinal Cancers: An Update.Journal of personalized medicine · 2022Review
- Human organoids in basic research and clinical applications.Signal transduction and targeted therapy · 2022Review
- Goblet cell hyperplasia is not epithelial-autonomous in the Cftr knockout intestine.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.Frontiers in cell and developmental biology · 2021Article
- Current Trends and Research Topics Regarding Intestinal Organoids: An Overview Based on Bibliometrics.Frontiers in cell and developmental biology · 2021Review
- Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells.Current protocols in immunology · 2020Article
- Gastrointestinal organoids: a next-generation tool for modeling human development.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Cystic Fibrosis, CFTR, and Colorectal Cancer.International journal of molecular sciences · 2020Review
- Enteroids expressing a disease-associated mutant of EpCAM are a model for congenital tufting enteropathy.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Role of ion channels in gastrointestinal cancer.World journal of gastroenterology · 2019Review
- Copper Transport and Disease: What Can We Learn from Organoids?Annual review of nutrition · 2019Review
- A BAC Transgene Expressing Human CFTR under Control of Its Regulatory Elements Rescues Cftr Knockout Mice.Scientific reports · 2019Article
- Tales from the crypt: new insights into intestinal stem cells.Nature reviews. Gastroenterology & hepatology · 2019Review
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Physiological studies of intact crypt epithelium have been limited by problems of accessibility in vivo and dedifferentiation in standard primary culture. Investigations of murine intestinal stem cells have recently yielded a primary intestinal culture in three-dimensional gel suspension that recapitulates crypt structure and epithelial differentiation (Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, Van Es JH, Abo A, Kujala P, Peters PJ, Clevers H. Nature 459: 262-265, 2009). We investigated the utility of murine intestinal crypt cultures (termed "enteroids") for physiological studies of crypt epithelium by focusing on the transport activity of the cystic fibrosis transmembrane conductance regulator Cftr. Enteroids had multiple crypts with well-differentiated goblet and Paneth cells that degranulated on exposure to the muscarinic agonist carbachol. Modified growth medium provided a crypt proliferation rate, as measured by 5-ethynyl-2'-deoxyuridine labeling, which was similar to proliferation in vivo. Immunoblots demonstrated equivalent Cftr expression in comparisons of freshly isolated crypts with primary and passage 1 enteroids. Apparent enteroid differences in mRNA expression of other transporters were primarily associated with villous epithelial contamination of freshly isolated crypts. Microelectrode analysis revealed cAMP-stimulated membrane depolarization in enteroid epithelium from wild-type (WT) but not Cftr knockout (KO) mice. Morphological and microfluorimetric studies, respectively, demonstrated Cftr-dependent cell shrinkage and lower intracellular pH in WT enteroid epithelium in contrast to Cftr KO epithelium or WT epithelium treated with Cftr inhibitor 172. We conclude that crypt epithelium of murine enteroids exhibit Cftr expression and activity that recapitulates crypt epithelium in vivo. Enteroids provide a primary culture model that is suitable for physiological studies of regenerating crypt epithelium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.