ArticlePloS one2012
Quantum dots do not affect the behaviour of mouse embryonic stem cells and kidney stem cells and are suitable for short-term tracking.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of cellular toxicity for cadmium-containing quantum dots.Nature nanotechnology · 2016Pooled it
- High-resolution in vivo kinematic tracking with customized injectable fluorescent nanoparticles.Science advances · 2025Article
- A Review of in vivo Toxicity of Quantum Dots in Animal Models.International journal of nanomedicine · 2023Review
- Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.PloS one · 2022Article
- Skin-derived mesenchymal stem cells as quantum dot vehicles to tumors.International journal of nanomedicine · 2017Article
- Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery.Beilstein journal of nanotechnology · 2017Article
- Biodistribution and Clearance of Human Mesenchymal Stem Cells by Quantitative Three-Dimensional Cryo-Imaging After Intravenous Infusion in a Rat Lung Injury Model.Stem cells translational medicine · 2016Article
- Quantum dots induce heat shock-related cytotoxicity at intracellular environment.In vitro cellular & developmental biology. Animal · 2014Article
- Seeing stem cells at work in vivo.Stem cell reviews and reports · 2014Review
- Quantum Dots Do Not Alter the Differentiation Potential of Pancreatic Stem Cells and Are Distributed Randomly among Daughter Cells.International journal of cell biology · 2013Article
- Article
- Personalized nanomedicine advancements for stem cell tracking.Advanced drug delivery reviews · 2012Review
- Probing stem cell behavior using nanoparticle-based approaches.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Quantum dots (QDs) are small nanocrystals widely used for labelling cells in order to enable cell tracking in complex environments in vitro, ex vivo and in vivo. They present many advantages over traditional fluorescent markers as they are resistant to photobleaching and have narrow emission spectra. Although QDs have been used effectively in cell tracking applications, their suitability has been questioned by reports showing they can affect stem cell behaviour and can be transferred to neighbouring cells. Using a variety of cellular and molecular biology techniques, we have investigated the effect of QDs on the proliferation and differentiation potential of two stem cell types: mouse embryonic stem cells and tissue-specific stem cells derived from mouse kidney. We have also tested if QDs released from living or dead cells can be taken up by neighbouring cells, and we have determined if QDs affect the degree of cell-cell fusion; this information is critical in order to assess the suitability of QDs for stem cell tracking. We show here that QDs have no effect on the viability, proliferation or differentiation potential of the two stem cell types. Furthermore, we show that the extent of transfer of QDs to neighbouring cells is <4%, and that QDs do not increase the degree of cell-cell fusion. However, although the QDs have a high labelling efficiency (>85%), they are rapidly depleted from both stem cell populations. Taken together, our results suggest that QDs are effective cell labelling probes that are suitable for short-term stem cell tracking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.