ArticlePloS one2012
Construction of a baculovirus-silkworm multigene expression system and its application on producing virus-like particles.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Chemosensory protein 3 is a brain host factor for the induction of enhanced-locomotory activity in the BmNPV-silkworm infection model.PLoS pathogens · 2025Article
- Immunogenicity of Virus-like Particles Based on VP1 Protein of Bovine Norovirus.Veterinary sciences · 2025Article
- Biotechnological Interventions for the Production of Subunit Vaccines Against Group A Rotavirus.Cell biochemistry and function · 2024Review
- Expression and Purification of Porcine Rotavirus Structural Proteins in Silkworm Larvae as a Vaccine Candidate.Molecular biotechnology · 2023Article
- Potential Proteins Interactions withInsects · 2022Article
- Article
- Article
- Sirt5 Inhibits BmNPV Replication by Promoting a Relish-Mediated Antiviral Pathway inFrontiers in immunology · 2022Article
- Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism.Viruses · 2021Article
- A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons.MicrobiologyOpen · 2020Article
- Transcriptional response of immune-related genes after endogenous expression of VP1 and exogenous exposure to VP1-based VLPs and CPV virions in lepidopteran cell lines.Molecular genetics and genomics : MGG · 2019Article
- An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus.Molecular genetics and genomics : MGG · 2019Article
- Construction of a highly efficient display system for baculovirus and its application on multigene co-display.Molecular genetics and genomics : MGG · 2018Article
- Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) Induces Host Cell Autophagy to Benefit Infection.Viruses · 2017Article
- Production of human type II collagen using an efficient baculovirus-silkworm multigene expression system.Molecular genetics and genomics : MGG · 2016Article
- A Highly Efficient and Simple Construction Strategy for Producing Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus.PloS one · 2016Article
- Chimaeric virus-like particles derived from consensus genome sequences of human rotavirus strains co-circulating in Africa.PloS one · 2014Article
- Construction and characterization of virus-like particles: a review.Molecular biotechnology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new baculovirus-silkworm multigene expression system named Bombyx mori MultiBac is developed and described here, by which multiple expression cassettes can be introduced into the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome efficiently. The system consists of three donor vectors (pCTdual, pRADM and pUCDMIG) and an invasive diaminopimelate (DAP) auxotrophic recipient E. coli containing BmNPV-Bacmid (BmBacmid) with a homologous recombination region, an attTn7 site and a loxp site. Two genes carried by pCTdual are firstly inserted into BmBacmid by homologous recombination, while the other eight genes in pRADM and pUCDMIG are introduced into BmBacmid through Tn7 transposition and cre-loxp recombination. Then the invasive and DAP auxotrophic E. coli carrying recombinant BmBacmid is directly injected into silkworm for expressing heterologous genes in larvae or pupae. Three structural genes of rotavirus and three fluorescent genes have been simultaneously expressed in silkworm larvae using our new system, resulting in the formation of virus-like particles (VLPs) of rotavirus and the color change of larvae. The VLPs were purified from hemolymph by ultracentrifugation using CsCl gradients, with a yield of 12.7 µg per larva. For the great capacity of foreign genes and the low cost of feeding silkworm, this high efficient BmMultiBac expression system provides a suitable platform to produce VLPs or protein complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.