ArticlePloS one2012
Isolation of a 97-kb minimal essential MHC B locus from a new reverse-4D BAC library of the golden pheasant.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Reconstructing Macroevolutionary Patterns in Avian MHC Architecture With Genomic Data.Frontiers in genetics · 2022Article
- Long-Read Genome Assemblies Reveal Extraordinary Variation in the Number and Structure of MHC Loci in Birds.Genome biology and evolution · 2021Article
- Cape Feather Coloration Signals Different Genotypes of the Most Polymorphic MHC Locus in Male Golden Pheasants (Animals : an open access journal from MDPI · 2021Article
- Molecular Characterization of MHC Class I Genes in Four Species of theBioMed research international · 2021Article
- Review
- 100 million years of multigene family evolution: origin and evolution of the avian MHC class IIB.BMC genomics · 2017Article
- Structure and polymorphisms of the major histocompatibility complex in the Oriental stork, Ciconia boyciana.Scientific reports · 2017Article
- Balancing selection and recombination as evolutionary forces caused population genetic variations in golden pheasant MHC class I genes.BMC evolutionary biology · 2016Article
- Signatures of Crested Ibis MHC Revealed by Recombination Screening and Short-Reads Assembly Strategy.PloS one · 2016Article
- Article
- Genomic organization of the crested ibis MHC provides new insight into ancestral avian MHC structure.Scientific reports · 2015Article
- The first report of a Pelecaniformes defensin cluster: characterization of β-defensin genes in the crested ibis based on BAC libraries.Scientific reports · 2014Article
- Structure and polymorphism of the major histocompatibility complex class II region in the Japanese Crested Ibis, Nipponia nippon.PloS one · 2014Article
- Comparative genome analyses reveal distinct structure in the saltwater crocodile MHC.PloS one · 2014Article
- Defense genes missing from the flight division.Developmental and comparative immunology · 2013Review
- Greater prairie chickens have a compact MHC-B with a single class IA locus.Immunogenetics · 2013Article
- Sequencing of the core MHC region of black grouse (Tetrao tetrix) and comparative genomics of the galliform MHC.BMC genomics · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial artificial chromosome (BAC) system is widely used in isolation of large genomic fragments of interest. Construction of a routine BAC library requires several months for picking clones and arraying BACs into superpools in order to employ 4D-PCR to screen positive BACs, which might be time-consuming and laborious. The major histocompatibility complex (MHC) is a cluster of genes involved in the vertebrate immune system, and the classical avian MHC-B locus is a minimal essential one, occupying a 100-kb genomic region. In this study, we constructed a more effective reverse-4D BAC library for the golden pheasant, which first creates sub-libraries and then only picks clones of positive sub-libraries, and identified several MHC clones within thirty days. The full sequencing of a 97-kb reverse-4D BAC demonstrated that the golden pheasant MHC-B locus contained 20 genes and showed good synteny with that of the chicken. The notable differences between these two species were the numbers of class II B loci and NK genes and the inversions of the TAPBP gene and the TAP1-TAP2 region. Furthermore, the inverse TAP2-TAP1 was unique in the golden pheasant in comparison with that of chicken, turkey, and quail. The newly defined genomic structure of the golden pheasant MHC will give an insight into the evolutionary history of the avian MHC.
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