ArticleBiomarker insights2012
Overcoming the effects of matrix interference in the measurement of urine protein analytes.
Article in Biomarker insights, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Associations of Serum Uromodulin and Urinary Epidermal Growth Factor with Measured Glomerular Filtration Rate and Interstitial Fibrosis in Kidney Transplantation.American journal of nephrology · 2022Trial
- Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation.PloS one · 2016Trial
- Case-Based Analysis of Pre-analytical and Analytical Non-Conformities in Urinary Protein Testing.EJIFCC · 2026Article
- Lithographically defined encoded magnetic heterostructures for the targeted screening of kidney cancer.Nanoscale advances · 2023Article
- Detection and Quantification of the Capsular Polysaccharide of Burkholderia pseudomallei in Serum and Urine Samples from Melioidosis Patients.Microbiology spectrum · 2022Article
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- A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable UropathogenicFrontiers in cellular and infection microbiology · 2022Article
- Urinary immunoglobulins in viral diagnosis: An overview.The Indian journal of medical research · 2022Review
- Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays.ACS sensors · 2021Article
- A New Method for Improving Extraction Efficiency and Purity of Urine and Plasma Cell-Free DNA.Diagnostics (Basel, Switzerland) · 2021Article
- Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads.Biosensors · 2020Article
- Biomarkers for tuberculosis: the case for lipoarabinomannan.ERJ open research · 2019Review
- Urinalysis findings and urinary kidney injury biomarker concentrations.BMC nephrology · 2017Article
- Development of Biomarker Models to Predict Outcomes in Lupus Nephritis.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- Evaluation of 32 urine biomarkers to predict the progression of acute kidney injury after cardiac surgery.Kidney international · 2014Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Using multiplex bead assays to measure urine proteins has a great potential for biomarker discovery, but substances in urine (the matrix) can interfere with assay measurements. By comparing the recovery of urine spiked with known quantities of several common analytes, this study demonstrated that the urine matrix variably interfered with the accurate measurement of low abundance proteins. Dilution of the urine permitted a more accurate measure of these proteins, equivalent to the standard dilution technique when the diluted analytes were above the limits of detection of the assay. Therefore, dilution can be used as an effective technique for over-coming urine matrix effects in urine immunoassays. These results may be applicable to other biological fluids in which matrix components interfere with assay performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.