ArticleJournal of clinical microbiology2012
Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Emerging Endemic Area for Blastomycosis, New York, USA, 2000-2024.Emerging infectious diseases · 2026Article
- Diagnosing Blastomycosis: A Review of Laboratory Methods and Clinical Utility.Journal of fungi (Basel, Switzerland) · 2025Review
- The Molecular Diagnosis of Invasive Fungal Diseases with a Focus on PCR.Diagnostics (Basel, Switzerland) · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Genome-level therapeutic targets identification and chimeric Vaccine designing against theHeliyon · 2024Article
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Article
- Blastomycosis: A Review of Mycological and Clinical Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Genitourinary Blastomycosis in a Young Male Patient: A Case Report and Review of Diagnostic Challenges.Case reports in infectious diseases · 2023Article
- Molecular Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Blastomycosis-Some Progress but Still Much to Learn.Journal of fungi (Basel, Switzerland) · 2022Review
- Recognition of Diagnostic Gaps for Laboratory Diagnosis of Fungal Diseases: Expert Opinion from the Fungal Diagnostics Laboratories Consortium (FDLC).Journal of clinical microbiology · 2021Article
- Diagnosis of Pulmonary Infections Due to Endemic Fungi.Diagnostics (Basel, Switzerland) · 2021Review
- Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis andJournal of clinical microbiology · 2021Article
- Current and New Perspectives in the Diagnosis of Blastomycosis and Histoplasmosis.Journal of fungi (Basel, Switzerland) · 2020Review
- Diagnosis of Breakthrough Fungal Infections in the Clinical Mycology Laboratory: An ECMM Consensus Statement.Journal of fungi (Basel, Switzerland) · 2020Article
- Radioimmunotherapy of Blastomycosis in a Mouse Model With a (1→3)-β-Glucans Targeting Antibody.Frontiers in microbiology · 2020Article
- Turning on virulence: Mechanisms that underpin the morphologic transition and pathogenicity ofVirulence · 2019Review
- Blastomyces Antigen Detection for Diagnosis and Management of Blastomycosis.Journal of clinical microbiology · 2015Article
- A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis.Journal of veterinary science · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.