ArticleToxicology in vitro : an international journal published in association with BIBRA2012
Variants of glutathione s-transferase pi 1 exhibit differential enzymatic activity and inhibition by heavy metals.
Article in Toxicology in vitro : an international journal published in association with BIBRA, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Influence of theInternational journal of environmental research and public health · 2026Article
- Genetic susceptibility to mercury in the amazon: A cross-sectional approach to snps analyses in exposed riverine populations.Genetics and molecular biology · 2026Article
- Effects of Turmeric on Manganese-induced Redox Imbalance in Testicular Tissues: Histo-micrometric and Biochemical analyses.Cell biochemistry and biophysics · 2025Article
- TheToxics · 2024Article
- Chronic Mercury Exposure andToxics · 2023Article
- Glutathione-related genetic polymorphisms are associated with mercury retention and nephrotoxicity in gold-mining settings of a Colombian population.Scientific reports · 2021Article
- Associations of Metabolic Genes (International journal of environmental research and public health · 2021Article
- Gene Variants Determine Placental Transfer of Perfluoroalkyl Substances (PFAS), Mercury (Hg) and Lead (Pb), and Birth Outcome: Findings From the UmMuKi Bratislava-Vienna Study.Frontiers in genetics · 2021Article
- Article
- Glutathione antioxidant system and methylmercury-induced neurotoxicity: An intriguing interplay.Biochimica et biophysica acta. General subjects · 2019Review
- The Influence of Red Meat on Colorectal Cancer Occurrence Is Dependent on the Genetic Polymorphisms of S-Glutathione Transferase Genes.Nutrients · 2019Article
- Cytology and molecular study for GSTP1 effect on asthma Iraqi patients.Clinical and molecular allergy : CMA · 2019Article
- Maternal polymorphisms in glutathione-related genes are associated with maternal mercury concentrations and early child neurodevelopment in a population with a fish-rich diet.Environment international · 2018Article
- Editor's Highlight: Glutathione S-Transferase Activity Moderates Methylmercury Toxicity During Development in Drosophila.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Unusual gastrointestinal and cutaneous toxicities by bleomycin, etoposide, and cisplatin: a case report with pharmacogenetic analysis to personalize treatment.The EPMA journal · 2017Article
- Genetic Aspects of Susceptibility to Mercury Toxicity: An Overview.International journal of environmental research and public health · 2017Review
- Genetic polymorphisms are associated with hair, blood, and urine mercury levels in the American Dental Association (ADA) study participants.Environmental research · 2016Article
- A global perspective on the influence of environmental exposures on the nervous system.Nature · 2015Review
- Effects of Total Ginsenosides on the Feeding Behavior and Two Enzymes Activities of Mythimna separata (Walker) Larvae.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- A positive correlation between mercury and oxidative stress-related gene expression (GPX3 and GSTM3) is measured in female Double-crested Cormorant blood.Ecotoxicology (London, England) · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nonsynonymous single nucleotide polymorphisms in glutathione s-transferase pi 1 (GSTP1; Ile/Val 105, Ala/Val 114) have been associated with altered toxicant metabolism in epidemiological cohorts. We explored the impact of GSTP1 genotype on enzyme kinetics and heavy metal inhibition in vitro. Four GSTP1 allozymes (105/114: Ile/Ala, Val/Ala, Ile/Val, Val/Val) were expressed in and purified from Escherichia coli. Enzyme activity assays quantifying the rate of glutathione conjugation with 1-chloro-2,4-dinitrobenzene (CDNB) revealed significant differences in kinetic parameters depending on genotype (p<0.01). Allozymes with Ile105 had better catalytic efficiency and greater affinity for CDNB (mean ± SEM: Ile105 Ala114 K(m)=0.33 ± 0.07 mM vs. Val105 Ala114 K(m)=1.15 ± 0.07 mM). Inhibition of GSTP1 activity by heavy metals was assessed following treatment with mercury (inorganic-HgCl(2), methylmercury-MeHg), selenium, cadmium, lead, arsenic, and manganese. All allozymes were inhibited by HgCl(2) (IC(50) range: 24.1-172 μM), MeHg (93.9-480 μM), and selenium (43.7-62.8 μM). Genotype significantly influenced the potency of mercury with GSTP1 Ile105 Val114 the least sensitive and Val105 Ala114 the most sensitive to inhibition by HgCl(2) and MeHg. Overall, genotype of two nonsynonymous polymorphisms in GSTP1 influenced enzyme kinetics pertaining to an electrophilic substrate and inhibition by two mercury species.
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