ArticleAnalytical chemistry2012
Metabolic cytometry: capillary electrophoresis with two-color fluorescence detection for the simultaneous study of two glycosphingolipid metabolic pathways in single primary neurons.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.Analytical chemistry · 2025Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- "Fix and Click" for Assay of Sphingolipid Signaling in Single Primary Human Intestinal Epithelial Cells.Analytical chemistry · 2022Article
- Photoactivatable Reporter to Perform Multiplexed and Temporally Controlled Measurements of Kinase and Protease Activity in Single Cells.Analytical chemistry · 2021Article
- Preserving Single Cells in Space and Time for Analytical Assays.Trends in analytical chemistry : TRAC · 2020Article
- "Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative.The Analyst · 2019Article
- Design and Application of Sensors for Chemical Cytometry.ACS chemical biology · 2018Review
- Chemical fixation to arrest phospholipid signaling for chemical cytometry.Journal of chromatography. A · 2017Article
- Chemical cytometry of thiols using capillary zone electrophoresis-laser induced fluorescence and TMPAB-o-M, an improved fluorogenic reagent.The Analyst · 2016Article
- Fluorescently labelled glycans and their applications.Glycoconjugate journal · 2015Review
- Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Single-cell sphingosine kinase activity measurements in primary leukemia.Analytical and bioanalytical chemistry · 2014Article
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Single cell metabolic profiling of tumor mimics.Analytical chemistry · 2013Article
- Article
- Progress toward single cell metabolomics.Current opinion in biotechnology · 2013Review
- Capillary electrophoresis with three-color fluorescence detection for the analysis of glycosphingolipid metabolism.The Analyst · 2013Article
- Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2012Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Metabolic cytometry is a form of chemical cytometry wherein metabolic cascades are monitored in single cells. We report the first example of metabolic cytometry where two different metabolic pathways are simultaneously monitored. Glycolipid catabolism in primary rat cerebella neurons was probed by incubation with tetramethylrhodamine-labeled GM1 (GM1-TMR). Simultaneously, both catabolism and anabolism were probed by coincubation with BODIPY-FL labeled LacCer (LacCer-BODIPY-FL). In a metabolic cytometry experiment, single cells were incubated with substrate, washed, aspirated into a capillary, and lysed. The components were separated by capillary electrophoresis equipped with a two-spectral channel laser-induced fluorescence detector. One channel monitored fluorescence generated by the metabolic products produced from GM1-TMR and the other monitored the metabolic products produced from LacCer-BODIPY-FL. The metabolic products were identified by comparison with the mobility of a set of standards. The detection system produced at least 6 orders of magnitude dynamic range in each spectral channel with negligible spectral crosstalk. Detection limits were 1 zmol for BODIPY-FL and 500 ymol for tetramethylrhodamine standard solutions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.