Evidence map›Paper›PMID 22400073›Full record

ArticleInternational journal of clinical and experimental pathology2012

Pitfalls in immunohistochemistry--a recent example.

Alexander Riechers, Anja Katrin Bosserhoff

Abstract read
In one paragraph

Article in International journal of clinical and experimental pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Alexander RiechersInstitute of Pathology, University of Regensburg, Germany.
Anja Katrin Bosserhoff

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunohistochemistry is an important and valuable technique in many fields of research, although several common pitfalls can lead to wrong or misinterpreted results. A recently published study [1] claims that the protein MIA (melanoma inhibitory activity) is expressed in Purkinje cells in the cerebellum. Careful re-analysis resulted in negative results. Due to these results of our group we feel that this analysis could serve as example for the potential problems in immunohistochemistry caused by the combination of an unspecific antibody and the omission of evaluating control tissue samples.

Indexed as

ArtifactsDiagnostic ErrorsBiomarkers, TumorCerebellumExtracellular Matrix ProteinsFemaleFluorescent Antibody Technique, DirectHumansNeoplasm ProteinsPurkinje CellsBiomarkers, TumorExtracellular Matrix ProteinsMIA protein, humanNeoplasm ProteinsantibodyCD-RAPImmunohistochemistryMIAspecificity

Identifiers

PMID22400073
PMCPMC3294226

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.