ArticleDevelopment (Cambridge, England)2012
Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 86 citations in OpenAlex.
- Discrete 3' UTR sequence motifs regulate nanos RNA enrichment in Drosophila germ granules.Developmental biology · 2026Article
- A Novel BIRC6 Variant Impairs Apoptotic Regulation in Familial Premature Ovarian Insufficiency: Functional Validation in a CRISPR/Cas9 Zebrafish Model.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Somatic gene repression ensures physical segregation of germline and soma in Drosophila embryos.EMBO reports · 2026Article
- Evolving Lessons on Metazoan Primordial Germ Cells in Diversity and Development.Molecular reproduction and development · 2025Review
- Review
- Deciphering the genetic basis of sex differentiation in silver-lipped pearl oyster (Zoological research · 2025Article
- Insights intoBiomolecules · 2025Article
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- The journey of a generation: advances and promises in the study of primordial germ cell migration.Development (Cambridge, England) · 2024Article
- Functional annotation of a hugely expanded nanos repertoire in Lytechinus variegatus, the green sea urchin.Molecular reproduction and development · 2023Article
- Article
- Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
- Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?Biology open · 2021Article
- Dead-end (dnd) protein in fish-a review.Fish physiology and biochemistry · 2021Review
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes inFrontiers in endocrinology · 2021Article
- Mammalian germ cells are determined after PGC colonization of the nascent gonad.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline.PLoS genetics · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Nanos is expressed in multipotent cells, stem cells and primordial germ cells (PGCs) of organisms as diverse as jellyfish and humans. It functions together with Pumilio to translationally repress targeted mRNAs. Here we show by loss-of-function experiments that Xenopus Nanos1 is required to preserve PGC fate. Morpholino knockdown of maternal Nanos1 resulted in a striking decrease in PGCs and a loss of germ cells from the gonads. Lineage tracing and TUNEL staining reveal that Nanos1-deficient PGCs fail to migrate out of the endoderm. They appear to undergo apoptosis rather than convert to normal endoderm. Whereas normal PGCs do not become transcriptionally active until neurula, Nanos1-depleted PGCs prematurely exhibit a hyperphosphorylated RNA polymerase II C-terminal domain at the midblastula transition. Furthermore, they inappropriately express somatic genes characteristic of endoderm regulated by maternal VegT, including Xsox17α, Bix4, Mixer, GATA4 and Edd. We further demonstrate that Pumilio specifically binds VegT RNA in vitro and represses, along with Nanos1, VegT translation within PGCs. Repressed VegT RNA in wild-type PGCs is significantly less stable than VegT in Nanos1-depleted PGCs. Our data indicate that maternal VegT RNA is an authentic target of Nanos1/Pumilio translational repression. We propose that Nanos1 functions to translationally repress RNAs that normally specify endoderm and promote apoptosis, thus preserving the germline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.