ArticleGenome research2012
Identification of novel causative genes determining the complex trait of high ethanol tolerance in yeast using pooled-segregant whole-genome sequence analysis.
Article in Genome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed.
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- Yeast strain development and process intensification in high-gravity fermentation.FEMS yeast research · 2026Review
- The yeast Mkt1/Pbp1 complex promotes adaptive responses to respiratory growth.The Journal of cell biology · 2025Article
- Unveiling genetic anchors in saccharomyces cerevisiae: QTL mapping identifies IRA2 as a key player in ethanol tolerance and beyond.Molecular genetics and genomics : MGG · 2024Article
- Optimal trade-off between boosted tolerance and growth fitness during adaptive evolution of yeast to ethanol shocks.Biotechnology for biofuels and bioproducts · 2024Article
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Identification of genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous proteins.PLoS biology · 2022Article
- CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae.FEMS yeast research · 2022Article
- Fine Mapping and Functional Analysis of the GeneApplied and environmental microbiology · 2022Article
- Use of Bulk Segregant Analysis for Determining the Genetic Basis of Azole Resistance in the Opportunistic PathogenFrontiers in cellular and infection microbiology · 2022Article
- QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae.Biotechnology for biofuels · 2021Article
- Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Quantitative trait loci controlling agronomic and biochemical traits in Cannabis sativa.Genetics · 2021Article
- Stress modulation as a means to improve yeasts for lignocellulose bioconversion.Applied microbiology and biotechnology · 2021Review
- Identification of novel genes involved in neutral lipid storage by quantitative trait loci analysis of Saccharomyces cerevisiae.BMC genomics · 2021Article
- A Major Effect Gene Controlling Development and Pathogenicity inFrontiers in plant science · 2021Article
- The RNA-associated proteins MKT1 and MKT1L form alternative PBP1-containing complexes inThe Journal of biological chemistry · 2020Article
- The roles and applications of chaotropes and kosmotropes in industrial fermentation processes.World journal of microbiology & biotechnology · 2020Review
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
31 more citing papers are in PubMed but not listed here.
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13 authors.
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Abstract
High ethanol tolerance is an exquisite characteristic of the yeast Saccharomyces cerevisiae, which enables this microorganism to dominate in natural and industrial fermentations. Up to now, ethanol tolerance has only been analyzed in laboratory yeast strains with moderate ethanol tolerance. The genetic basis of the much higher ethanol tolerance in natural and industrial yeast strains is unknown. We have applied pooled-segregant whole-genome sequence analysis to map all quantitative trait loci (QTL) determining high ethanol tolerance. We crossed a highly ethanol-tolerant segregant of a Brazilian bioethanol production strain with a laboratory strain with moderate ethanol tolerance. Out of 5974 segregants, we pooled 136 segregants tolerant to at least 16% ethanol and 31 segregants tolerant to at least 17%. Scoring of SNPs using whole-genome sequence analysis of DNA from the two pools and parents revealed three major loci and additional minor loci. The latter were more pronounced or only present in the 17% pool compared to the 16% pool. In the locus with the strongest linkage, we identified three closely located genes affecting ethanol tolerance: MKT1, SWS2, and APJ1, with SWS2 being a negative allele located in between two positive alleles. SWS2 and APJ1 probably contained significant polymorphisms only outside the ORF, and lower expression of APJ1 may be linked to higher ethanol tolerance. This work has identified the first causative genes involved in high ethanol tolerance of yeast. It also reveals the strong potential of pooled-segregant sequence analysis using relatively small numbers of selected segregants for identifying QTL on a genome-wide scale.
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