ArticleLab on a chip2012
Digital LAMP in a sample self-digitization (SD) chip.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 185 citations in OpenAlex.
- PEG-mediated viscous crowding controls dynamics and quantitative range in recombinase polymerase amplification nucleation site analysis.Sensors & diagnostics · 2026Article
- Advances of Digital Detection for Foodborne Pathogens.Foods (Basel, Switzerland) · 2026Review
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries.Pathogens (Basel, Switzerland) · 2026Review
- Critical aspects of droplet digital reverse transcription loop-mediated isothermal amplification (ddRT-LAMP) for viral pathogens detection.Microsystems & nanoengineering · 2025Article
- Development and Validation of the MAST ISOPLEXInternational journal of molecular sciences · 2024Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Article
- All-In-One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Digital droplet RT-LAMP increases speed of SARS-CoV-2 viral RNA detection.Smart medicine · 2024Article
- Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria.Practical laboratory medicine · 2024Article
- STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads.ACS nano · 2023Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Review
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Picoliter Droplet Generation and Dense Bead-in-Droplet Encapsulation via Microfluidic Devices Fabricated via 3D Printed Molds.Micromachines · 2022Article
- Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis.Lab on a chip · 2022Article
- Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor.Biosensors · 2022Article
- Precision Medicine in Low- and Middle-Income Countries.Annual review of pathology · 2022Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
This paper describes the realization of digital loop-mediated DNA amplification (dLAMP) in a sample self-digitization (SD) chip. Digital DNA amplification has become an attractive technique to quantify absolute concentrations of DNA in a sample. While digital polymerase chain reaction is still the most widespread implementation, its use in resource-limited settings is impeded by the need for thermal cycling and robust temperature control. In such situations, isothermal protocols that can amplify DNA or RNA without thermal cycling are of great interest. Here, we accomplished the successful amplification of single DNA molecules in a stationary droplet array using isothermal digital loop-mediated DNA amplification. Unlike most (if not all) existing methods for sample discretization, our design allows for automated, loss-less digitization of sample volumes on-chip. We demonstrated accurate quantification of relative and absolute DNA concentrations with sample volumes of less than 2 μl. We assessed the homogeneity of droplet size during sample self-digitization in our device, and verified that the size variation was small enough such that straightforward counting of LAMP-active droplets sufficed for data analysis. We anticipate that the simplicity and robustness of our SD chip make it attractive as an inexpensive and easy-to-operate device for DNA amplification, for example in point-of-care settings.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.