ArticleMolecular biology of the cell2012
Localized accumulation of tubulin during semi-open mitosis in the Caenorhabditis elegans embryo.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Motorless transport of microtubules along tubulin, RanGTP, and salt gradients.Nature communications · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure.Journal of cell science · 2023Article
- Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure.bioRxiv : the preprint server for biology · 2023Article
- Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.Scientific reports · 2022Article
- Cortical microtubule pulling forces contribute to the union of the parental genomes in theeLife · 2022Article
- Nuclear envelope remodelling during mitosis.Current opinion in cell biology · 2021Review
- Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched Hyphae of an Oomycete Pathogen.mBio · 2019Article
- Article
- Mitotic Cell Division inGenetics · 2019Review
- Dynein pulling forces counteract lamin-mediated nuclear stability during nuclear envelope repair.Molecular biology of the cell · 2018Article
- Cell Biology of the Caenorhabditis elegans Nucleus.Genetics · 2017Review
- Mixing and matching nuclear envelope remodeling and spindle assembly strategies in the evolution of mitosis.Current opinion in cell biology · 2016Review
- Caenorhabditis elegans polo-like kinase PLK-1 is required for merging parental genomes into a single nucleus.Molecular biology of the cell · 2015Article
- Article
- An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.The Journal of cell biology · 2015Article
- Review
- A computational model for the formation of lamin-B mitotic spindle envelope and matrix.Interface focus · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of microtubules inside the cell is controlled both spatially and temporally. During mitosis, microtubule assembly must be activated locally at the nascent spindle region for mitotic spindle assembly to occur efficiently. In this paper, we report that mitotic spindle components, such as free tubulin subunits, accumulated in the nascent spindle region, independent of spindle formation in the Caenorhabditis elegans embryo. This accumulation coincided with nuclear envelope permeabilization, suggesting that permeabilization might trigger the accumulation. When permeabilization was induced earlier by knockdown of lamin, tubulin also accumulated earlier. The boundaries of the region of accumulation coincided with the remnant nuclear envelope, which remains after nuclear envelope breakdown in cells that undergo semi-open mitosis, such as those of C. elegans. Ran, a small GTPase protein, was required for tubulin accumulation. Fluorescence recovery after photobleaching analysis revealed that the accumulation was accompanied by an increase in the immobile fraction of free tubulin inside the remnant nuclear envelope. We propose that this newly identified mechanism of accumulation of free tubulin-and probably of other molecules-at the nascent spindle region contributes to efficient assembly of the mitotic spindle in the C. elegans embryo.
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