ArticleJournal of virology2012
Development of an insect vector cell culture and RNA interference system to investigate the functional role of fijivirus replication protein.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 99 citations in OpenAlex.
- Psyllid Cysteine Cathepsins Directly Cleave the Outer Membrane Protein BamD of Citrus Huanglongbing Pathogen.Plant biotechnology journal · 2026Article
- The insect Toll pathway activates antibacterial immunity against the citrus Huanglongbing pathogen.Nature communications · 2026Article
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- Insect ribosome-rescuer Pelo-Hbs1 complex on sperm surface mediates paternal arbovirus transmission.Nature communications · 2024Article
- Arboviruses antagonize insect Toll antiviral immune signaling to facilitate the coexistence of viruses with their vectors.PLoS pathogens · 2024Article
- Autophagy mediates a direct synergistic interaction during co-transmission of two distinct arboviruses by insect vectors.Science China. Life sciences · 2023Article
- A Rice Receptor-like Protein Negatively Regulates Rice Resistance to Southern Rice Black-Streaked Dwarf Virus Infection.Viruses · 2023Article
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- Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect.PLoS pathogens · 2023Article
- A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain.Molecular plant pathology · 2023Article
- A nonstructural protein encoded by a rice reovirus induces an incomplete autophagy to promote viral spread in insect vectors.PLoS pathogens · 2022Article
- Polyamine-metabolizing enzymes are activated to promote the proper assembly of rice stripe mosaic virus in insect vectors.Stress biology · 2022Article
- Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- The Infection Route of Tomato Zonate Spot Virus in the Digestive System of Its Insect VectorFrontiers in microbiology · 2022Article
- Development of Nanobodies against Mal de Río Cuarto virus major viroplasm protein P9-1 for diagnostic sandwich ELISA and immunodetection.Scientific reports · 2021Article
- Southern rice black-streaked dwarf virus hijacks SNARE complex of its insect vector for its effective transmission to rice.Molecular plant pathology · 2021Article
- RNA NMolecular plant pathology · 2021Article
- A plant reovirus hijacks endoplasmic reticulum-associated degradation machinery to promote efficient viral transmission by its planthopper vector under high temperature conditions.PLoS pathogens · 2021Article
- The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission.PLoS pathogens · 2020Article
- Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro culture system of primary cells from white-backed planthopper, an insect vector of Southern rice black-streaked dwarf virus (SRBSDV), a fijivirus, was established to study replication of the virus. Viroplasms, putative sites of viral replication, contained the nonstructural viral protein P9-1, viral RNA, outer-capsid proteins, and viral particles in virus-infected cultured insect vector cells, as revealed by transmission electron and confocal microscopy. Formation of viroplasm-like structures in non-host insect cells upon expression of P9-1 suggested that the matrix of viroplasms observed in virus-infected cells was composed basically of P9-1. In cultured insect vector cells, knockdown of P9-1 expression due to RNA interference (RNAi) induced by synthesized double-stranded RNA (dsRNA) from the P9-1 gene strongly inhibited viroplasm formation and viral infection. RNAi induced by ingestion of dsRNA strongly abolished viroplasm formation, preventing efficient viral spread in the body of intact vector insects. All these results demonstrated that P9-1 was essential for viroplasm formation and viral replication. This system, combining insect vector cell culture and RNA interference, can further advance our understanding of the biological activities of fijivirus replication proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.