Evidence map›Paper›PMID 22397847›Full record

ArticleEuropean urology2012

Both immediate and delayed intracavernous injection of autologous adipose-derived stromal vascular fraction enhances recovery of erectile function in a rat model of cavernous nerve injury.

Xuefeng Qiu, Thomas M Fandel, Ludovic Ferretti, Maarten Albersen, Hazem Orabi, Haiyang Zhang, Guiting Lin, Ching-Shwun Lin, Tania Schroeder, Tom F Lue

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European urology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed, 2 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 2 syntheses or guidelines pooled it, 99 citations in OpenAlex.

  1. Pooled it
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  18. Adipose-derived stem cells modified byNeural regeneration research · 2020
    Article
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4 · The record

Corrections and comments

  • Erratum issued
    Orabi, Hazem [added]
5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Xuefeng QiuKnuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143-0738, USA.
Thomas M Fandel
Ludovic Ferretti
Maarten Albersen
Hazem Orabi
Haiyang Zhang
Guiting Lin
Ching-Shwun Lin
Tania Schroeder
Tom F Lue
University of California, San Francisco · USAmerican Systems (United States) · USNanjing Drum Tower Hospital · CN

Funding

Prevention and Treatment of ImpotenceR37DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2004 to 2013
$3.5M
TREATMENT AND PREVENTION OF IMPOTENCER01DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI LUE, TOM F · 1994 to 2001
$631k
NIDDK NIH HHS R01 DK045370NIDDK NIH HHS R37 DK045370
6 · The paper itself

Abstract

backgroundIntracavernous injection of cultured adipose-derived stem cells (ADSCs) effectively restores erectile function in cavernous nerve (CN)-injured rats when administered at the time of injury. However, culturing exposes ADSCs to the risk of contamination and dedifferentiation.

objectiveExplore the effect of uncultured autologous adipose-derived stromal vascular fraction (SVF) on improving erectile function in a rat model of CN injury when administered at the time of injury or 4 wk after injury. DESIGN, SETTING, AND

participantsEighty-nine male Sprague Dawley rats were randomly divided into four groups. CN injury or sham surgery was performed at the start of the study, and rats were treated with either SVF or vehicle. Functional testing and histologic analysis were performed 12 wk after CN crush or sham surgery.

interventionWe used intracavernous injection of saline immediately after CN crush (n=23), intracavernous injection of SVF immediately after CN crush (n=17), intracavernous injection of SVF 4 wk after CN crush (n=23), or sham surgery (n=26). OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: We studied intracavernous pressure (ICP) response to CN electrostimulation and performed histologic examination of midpenile cross-sections. Data were analyzed using one-way analysis of variance followed by the Tukey-Kramer test. RESULTS AND LIMITATIONS: Both immediate and delayed treatment with SVF resulted in a significantly increased ICP-to-mean arterial pressure ratio compared with the vehicle-treated group. Both immediate and delayed treatment with SVF significantly increased expression of neuronal nitric oxide synthase and neurofilament in dorsal penile nerves compared to the vehicle group. Furthermore, the smooth muscle-to-collagen ratio within the corpus cavernosum was significantly improved in both of the SVF groups compared to vehicle-treated rats. The main limitation of the study is the lack of determination of the SVF components.

conclusionsUncultured autologous SVF injected immediately or 4 wk after CN crush improved erectile function, promoted nerve regeneration, and prevented fibrosis of the corpus cavernosum following CN injury.

Indexed as

Adipose TissueAnimalsDisease Models, AnimalErectile DysfunctionMaleMuscle, SmoothNerve CrushNerve RegenerationNitric Oxide Synthase Type IPenile ErectionPenisPudendal NerveRatsRats, Sprague-DawleyRecovery of FunctionStromal CellsNitric Oxide Synthase Type I

Identifiers

PMID22397847
PMCPMC3542983
OpenAlexW2051696250

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.