ArticleBiochemistry2012
Toxoplasma gondii profilin acts primarily to sequester G-actin while formins efficiently nucleate actin filament formation in vitro.
Article in Biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Highly divergent apicomplexan cytoskeletons provide additional models for actin biology.The FEBS journal · 2025Review
- Myosin F controls actin organization and dynamics inMolecular biology of the cell · 2024Article
- Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography.Nature communications · 2023Article
- Sulfonylpiperazine compounds prevent Plasmodium falciparum invasion of red blood cells through interference with actin-1/profilin dynamics.PLoS biology · 2023Article
- Transcriptomic Differences between Free-Living and ParasiticMicroorganisms · 2022Article
- Marine gregarine genomes reveal the breadth of apicomplexan diversity with a partially conserved glideosome machinery.BMC genomics · 2022Article
- Dehydroepiandrosterone Effect onMicroorganisms · 2021Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Emerging Functions of Actins and Actin Binding Proteins in Trypanosomatids.Frontiers in cell and developmental biology · 2020Review
- Comparative and functional genomics of the protozoan parasite Babesia divergens highlighting the invasion and egress processes.PLoS neglected tropical diseases · 2019Article
- Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5.Innate immunity · 2018Article
- Gliding motility powers invasion and egress in Apicomplexa.Nature reviews. Microbiology · 2017Review
- A unique profilin-actin interface is important for malaria parasite motility.PLoS pathogens · 2017Article
- Dense granule trafficking in Toxoplasma gondii requires a unique class 27 myosin and actin filaments.Molecular biology of the cell · 2016Article
- Towards a molecular understanding of the apicomplexan actin motor: on a road to novel targets for malaria remedies?Acta crystallographica. Section F, Structural biology communications · 2015Review
- Host cell invasion by apicomplexan parasites: the junction conundrum.PLoS pathogens · 2014Review
- Synthetic chondramide A analogues stabilize filamentous actin and block invasion by Toxoplasma gondii.Journal of natural products · 2013Article
- Toxoplasma gondii myosin F, an essential motor for centrosomes positioning and apicoplast inheritance.The EMBO journal · 2013Article
- Combined action of nucleic acid-sensing Toll-like receptors and TLR11/TLR12 heterodimers imparts resistance to Toxoplasma gondii in mice.Cell host & microbe · 2013Article
- The unusual dynamics of parasite actin result from isodesmic polymerization.Nature communications · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Apicomplexan parasites employ gliding motility that depends on the polymerization of parasite actin filaments for host cell entry. Despite this requirement, parasite actin remains almost entirely unpolymerized at steady state; formation of filaments required for motility relies on a small repertoire of actin-binding proteins. Previous studies have shown that apicomplexan formins and profilin exhibit canonical functions on heterologous actins from higher eukaryotes; however, their biochemical properties on parasite actins are unknown. We therefore analyzed the impact of T. gondii profilin (TgPRF) and FH1-FH2 domains of two formin isoforms in T. gondii (TgFRM1 and TgFRM2) on the polymerization of T. gondii actin (TgACTI). Our findings based on in vitro assays demonstrate that TgFRM1-FH1-FH2 and TgFRM2-FH1-FH2 dramatically enhanced TgACTI polymerization in the absence of profilin, making them the sole protein factors known to initiate polymerization of this normally unstable actin. In addition, T. gondii formin domains were shown to both initiate polymerization and induce bundling of TgACTI filaments; however, they did not rely on TgPRF for these activities. In contrast, TgPRF sequestered TgACTI monomers, thus inhibiting polymerization even in the presence of formins. Collectively, these findings provide insight into the unusual control mechanisms of actin dynamics within the parasite.
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