ArticleThe Analyst2012
Quantum dot enabled detection of Escherichia coli using a cell-phone.
Article in The Analyst, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed.
- Advances in Portable Optical Microscopy Using Cloud Technologies and Artificial Intelligence for Medical Applications.Sensors (Basel, Switzerland) · 2024Review
- A lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria.iScience · 2022Article
- Prototype Smartphone-Based Device for Flow Cytometry with Immunolabeling via Supra-nanoparticle Assemblies of Quantum Dots.ACS measurement science au · 2022Article
- Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA.Scientific reports · 2021Article
- Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology-A Review.Foods (Basel, Switzerland) · 2021Review
- Applications of smartphone-based near-infrared (NIR) imaging, measurement, and spectroscopy technologies to point-of-care (POC) diagnostics.Journal of Zhejiang University. Science. B · 2021Review
- Transcription Factor Based Small-Molecule Sensing with a Rapid Cell Phone Enabled Fluorescent Bead Assay.Angewandte Chemie (International ed. in English) · 2020Article
- Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices.Diagnostics (Basel, Switzerland) · 2020Review
- Smartphone-based intraocular lens microscope.Indian journal of ophthalmology · 2020Article
- Recent Progress on the Electrochemical Biosensing ofBiosensors · 2020Review
- Hyperspectral Imaging in Environmental Monitoring: A Review of Recent Developments and Technological Advances in Compact Field Deployable Systems.Sensors (Basel, Switzerland) · 2019Review
- Cell phone based colorimetric analysis for point-of-care settings.The Analyst · 2019Article
- Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity.ACS omega · 2019Article
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Smartphone-based clinical diagnostics: towards democratization of evidence-based health care.Journal of internal medicine · 2019Review
- Identification of pathogenic bacteria in complex samples using a smartphone based fluorescence microscope.RSC advances · 2018Article
- "The Smartphone's Guide to the Galaxy": In Situ Analysis in Space.Biosensors · 2018Review
- Microfluidic Based Optical Microscopes on Chip.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Review
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We report a cell-phone based Escherichia coli (E. coli) detection platform for screening of liquid samples. In this compact and cost-effective design attached to a cell-phone, we utilize anti-E. coli O157:H7 antibody functionalized glass capillaries as solid substrates to perform a quantum dot based sandwich assay for specific detection of E. coli O157:H7 in liquid samples. Using battery-powered inexpensive light-emitting-diodes (LEDs) we excite/pump these labelled E. coli particles captured on the capillary surface, where the emission from the quantum dots is then imaged using the cell-phone camera unit through an additional lens that is inserted between the capillary and the cell-phone. By quantifying the fluorescent light emission from each capillary tube, the concentration of E. coli in the sample is determined. We experimentally confirmed the detection limit of this cell-phone based fluorescent imaging and sensing platform as ∼5 to 10 cfu mL(-1) in buffer solution. We also tested the specificity of this E. coli detection platform by spiking samples with different species (e.g., Salmonella) to confirm that non-specific binding/detection is negligible. We further demonstrated the proof-of-concept of our approach in a complex food matrix, e.g., fat-free milk, where a similar detection limit of ∼5 to 10 cfu mL(-1) was achieved despite challenges associated with the density of proteins that exist in milk. Our results reveal the promising potential of this cell-phone enabled field-portable and cost-effective E. coli detection platform for e.g., screening of water and food samples even in resource limited environments. The presented platform can also be applicable to other pathogens of interest through the use of different antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.