ArticlePLoS pathogens2012
Phosphorylcholine allows for evasion of bactericidal antibody by Haemophilus influenzae.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- The cGAS-STING signaling pathway in the regulation of pulmonary infections: a systematic review.Frontiers in cellular and infection microbiology · 2025Pooled it
- Effects of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D-conjugate vaccine on nasopharyngeal bacterial colonization in young children: a randomized controlled trial.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2013Trial
- Phase variable acetylation of lipooligosaccharide modifies antibody production and opsonophagocytic killing of non-typeableiScience · 2023Article
- Article
- Genetic Adaptation and Acquisition of Macrolide Resistance in Haemophilus spp. during Persistent Respiratory Tract Colonization in Chronic Obstructive Pulmonary Disease (COPD) Patients Receiving Long-Term Azithromycin Treatment.Microbiology spectrum · 2023Article
- Non-typeableFrontiers in cellular and infection microbiology · 2023Article
- Impact of Phosphorylcholine Expression on the Adherence and Invasion ofMicroorganisms · 2022Article
- Zwitterionic Polymer Coatings Enhance Gold Nanoparticle Stability and Uptake in Various Biological Environments.The AAPS journal · 2022Article
- Choline Kinase: An Unexpected Journey for a Precision Medicine Strategy in Human Diseases.Pharmaceutics · 2021Review
- Duodenal Metatranscriptomics to Define Human and Microbial Functional Alterations Associated with Severe Obesity: A Pilot Study.Microorganisms · 2020Article
- Antibody Binding and Complement-Mediated Killing of Invasive Haemophilus influenzae Isolates from Spain, Portugal, and the Netherlands.Infection and immunity · 2020Article
- Impact of the changes in bacterial outer membrane structure on the anti-bacterial activity of zinc oxide nanoparticles.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2020Article
- ChoK-ing the Pathogenic Bacteria: Potential of Human Choline Kinase Inhibitors as Antimicrobial Agents.BioMed research international · 2020Review
- Translating Recent Microbiome Insights in Otitis Media into Probiotic Strategies.Clinical microbiology reviews · 2019Review
- The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis.Nature communications · 2019Article
- Platelet activating factor receptor regulates colitis-induced pulmonary inflammation through the NLRP3 inflammasome.Mucosal immunology · 2019Article
- Nontypeable Haemophilus influenzae Invasive Blood Isolates Are Mainly Phosphorylcholine Negative and Show Decreased Complement-Mediated Killing That Is Associated with Lower Binding of IgM and CRP in Comparison to Colonizing Isolates from the Oropharynx.Infection and immunity · 2019Article
- Choline Kinase Emerges as a Promising Drug Target in Gram-Positive Bacteria.Frontiers in microbiology · 2019Article
- Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1.Medical microbiology and immunology · 2018Article
- Phosphocholine-Modified Lipooligosaccharides ofMolecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The human pathogen Haemophilus influenzae has the ability to quickly adapt to different host environments through phase variation of multiple structures on its lipooligosaccharide (LPS), including phosphorylcholine (ChoP). During colonization with H. influenzae, there is a selection for ChoP+ phase variants. In a murine model of nasopharyngeal colonization, this selection is lost in the absence of adaptive immunity. Based on previous data highlighting the importance of natural antibody in limiting H. influenzae colonization, the effect of ChoP expression on antibody binding and its bactericidal activity was investigated. Flow cytometric analysis revealed that ChoP+ phase variants had decreased binding of antibody to LPS epitopes compared to ChoP- phase variants. This difference in antibody binding correlated with increased survival of ChoP+ phase variants in the presence of antibody-dependent, complement-mediated killing. ChoP+ phase variants were also more resistant to trypsin digestion, suggesting a general effect on the physical properties of the outer membrane. Moreover, ChoP-mediated protection against antibody binding correlated with increased resilience of outer membrane integrity. Collectively, these data suggest that ChoP expression provides a selective advantage during colonization through ChoP-mediated effects on the accessibility of bactericidal antibody to the cell surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.