ArticleDiabetes2012
A novel method to measure glucose uptake and myosin heavy chain isoform expression of single fibers from rat skeletal muscle.
Article in Diabetes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Evaluation of the toxicity potential of exercise and atorvastatin/metformin combination therapy on STZ-diabetic rats.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Epidemiological, mechanistic, and practical bases for assessment of cardiorespiratory fitness and muscle status in adults in healthcare settings.European journal of applied physiology · 2023Review
- Skeletal muscle cell-specific differences in type 2 diabetes.Cellular and molecular life sciences : CMLS · 2022Article
- Measurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.Acta physiologica (Oxford, England) · 2022Article
- Differential effect of canagliflozin, a sodium-glucose cotransporter 2 (SGLT2) inhibitor, on slow and fast skeletal muscles from nondiabetic mice.The Biochemical journal · 2022Article
- In vivo glucoregulation and tissue-specific glucose uptake in female Akt substrate 160 kDa knockout rats.PloS one · 2020Article
- Fiber type-selective exercise effects on AS160 phosphorylation.American journal of physiology. Endocrinology and metabolism · 2019Article
- Skeletal muscle fiber type-selective effects of acute exercise on insulin-stimulated glucose uptake in insulin-resistant, high-fat-fed rats.American journal of physiology. Endocrinology and metabolism · 2019Article
- Low survival rate and muscle fiber-dependent aging effects in the McArdle disease mouse model.Scientific reports · 2019Article
- Involvement of the protein kinase Akt2 in insulin-stimulated Rac1 activation leading to glucose uptake in mouse skeletal muscle.PloS one · 2019Article
- Whole body glucoregulation and tissue-specific glucose uptake in a novel Akt substrate of 160 kDa knockout rat model.PloS one · 2019Article
- ASAS-SSR Triennnial Reproduction Symposium: Looking Back and Moving Forward-How Reproductive Physiology has Evolved: Fetal origins of impaired muscle growth and metabolic dysfunction: Lessons from the heat-stressed pregnant ewe.Journal of animal science · 2018Review
- Effects of Calorie Restriction and Fiber Type on Glucose Uptake and Abundance of Electron Transport Chain and Oxidative Phosphorylation Proteins in Single Fibers from Old Rats.The journals of gerontology. Series A, Biological sciences and medical sciences · 2017Article
- High-Fat Diet-Induced Insulin Resistance in Single Skeletal Muscle Fibers is Fiber Type Selective.Scientific reports · 2017Article
- Resistance training to improve type 2 diabetes: working toward a prescription for the future.Nutrition & metabolism · 2017Review
- Development of a high-throughput method for real-time assessment of cellular metabolism in intact long skeletal muscle fibre bundles.The Journal of physiology · 2016Article
- Novel single skeletal muscle fiber analysis reveals a fiber type-selective effect of acute exercise on glucose uptake.American journal of physiology. Endocrinology and metabolism · 2016Article
- Protein Phosphatase 1-α Regulates AS160 Ser588 and Thr642 Dephosphorylation in Skeletal Muscle.Diabetes · 2016Article
- Intrauterine growth-restricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Fiber type effects on contraction-stimulated glucose uptake and GLUT4 abundance in single fibers from rat skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Skeletal muscle includes many individual fibers with diverse phenotypes. A barrier to understanding muscle glucose uptake at the cellular level has been the absence of a method to measure glucose uptake by single fibers from mammalian skeletal muscle. This study's primary objective was to develop a procedure to measure glucose uptake by single fibers from rat skeletal muscle. Rat epitrochlearis muscles were incubated ex vivo with [(3)H]-2-deoxy-d-glucose, with or without insulin or AICAR, before isolation of ~10-30 single fibers from each muscle. Fiber type (myosin heavy chain [MHC] isoform) and glucose uptake were determined for each single fiber. Insulin-stimulated glucose uptake (which was cytochalasin B inhibitable) varied according to MHC isoform expression, with ~2-fold greater values for IIA versus IIB or IIX fibers and ~1.3-fold greater for hybrid (IIB/X) versus IIB fibers. In contrast, AICAR-stimulated glucose uptake was ~1.5-fold greater for IIB versus IIA fibers. A secondary objective was to assess insulin resistance of single fibers from obese versus lean Zucker rats. Genotype differences were observed for insulin-stimulated glucose uptake and inhibitor κB (IκB)-β abundance in single fibers (obese less than lean), with decrements for glucose uptake (44-58%) and IκB-β (25-32%) in each fiber type. This novel method creates a unique opportunity for future research focused on understanding muscle glucose uptake at the cellular level.
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Registered trials
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