ArticleParasitology research2012
Molecular characterization of Acanthamoeba isolated in water treatment plants and comparison with clinical isolates.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global prevalence of potentially pathogenic free-living amoebae in sewage and sewage-related environments-systematic review with meta-analysis.Parasitology research · 2024Pooled it
- Molecular identification and genotype distribution of Acanthamoeba in environmental water.Irish journal of medical science · 2026Article
- Complicated Diagnosis and Treatment of Rare PainlessJournal of clinical medicine · 2025Article
- High Diversity and Prevalence of Potentially Pathogenic Free-Living Amoebae in Water Sources from Castilla y León, Spain.Pathogens (Basel, Switzerland) · 2025Article
- Assessment of the Presence of Free-Living Amoebae in Soil Samples from the Northwest Region of Spain Using Culture and Molecular Assays.Microorganisms · 2025Article
- Monitoring potentially pathogenic protists in sewage sludge using Metataxonomics.Food and waterborne parasitology · 2023Article
- Article
- Article
- Extracellular Vesicles from the ProtozoaBioengineering (Basel, Switzerland) · 2019Review
- Article
- Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China.The Korean journal of parasitology · 2018Article
- Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.BMC microbiology · 2018Article
- Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Pathogens (Basel, Switzerland) · 2018Article
- Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach.Parasites & vectors · 2018Article
- The Influence ofFrontiers in microbiology · 2018Article
- Molecular and Morphometric Characterization ofJundishapur journal of microbiology · 2016Article
- Article
- Occurrence of pathogenic Acanthamoeba genotypes in nasal swabs of cancer patients in Iran.Parasitology research · 2015Article
- Isolation and molecular characterization of free-living amoebae from different water sources in Italy.International journal of environmental research and public health · 2015Article
- Genotypic characterization of amoeba isolated from Acanthamoeba keratitis in Poland.Parasitology research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 116 samples (44 clinical specimens and 72 environmental samples) have been analyzed for the presence of Acanthamoeba. The environmental samples (ESs) were collected from four drinking water treatment plants (DWTP, n=32), seven wastewater treatment plants (n=28), and six locations of influence (n=12) on four river basins from the central area of Spain (winter-spring 2008). Water samples were concentrated by using the IDEXX Filta-Max(®) system. Acanthamoeba was identified in 65 of the 72 ESs by culture isolation (90.3%) and 63 by real-time PCR (87.5%), resulting in all sampling points (100%) positive for Acanthamoeba when considering both techniques and all the time period analyzed. Nine of the 44 clinical specimens were positive for Acanthamoeba. Seventeen Acanthamoeba strains (eight from four DWTP and nine from clinical samples) were also established in axenic-PYG medium. Twenty-four of the ESs and the 17 Acanthamoeba sp. strains were genotyped as T4/1, T4/8, and T4/9. The eight strains isolated from the DWTP samples were inoculated in nude mouse to ascertain their potential pathogenicity in this model. Animals that were inoculated died or showed central nervous system symptoms 9 days post-inoculation. Examination of immunofluorescence-stained brain and lung tissue sections showed multiple organisms invading both tissues, and re-isolation of throphozoites was successful in these tissues of all infected animals. For the first time, potentially pathogenic Acanthamoeba T4 has been detected in 100% of different types of water samples including tap water and sewage effluents in the central area of Spain suggesting a potential health threat for humans especially for the contact lens wearers.
Indexed as
Identifiers
22395660What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.