ArticleThe Plant cell2012
Pulsing of membrane potential in individual mitochondria: a stress-induced mechanism to regulate respiratory bioenergetics in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 120 citations in OpenAlex.
- Electron transport reactions of cyanobacterial photosynthesis and state-of-the-art in vivo measurement techniques.Plant physiology · 2026Review
- MIRO1-mediated mitochondrial fusion is required for stomatal immunity in Arabidopsis.Nature plants · 2026Article
- Perception and processing of stress signals by plant mitochondria.The Plant journal : for cell and molecular biology · 2024Review
- The Switching of the Type of a ROS Signal from Mitochondria: The Role of Respiratory Substrates and Permeability Transition.Antioxidants (Basel, Switzerland) · 2024Article
- Review
- Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses.International journal of molecular sciences · 2022Review
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Empowering roots-Some current aspects of root bioenergetics.Frontiers in plant science · 2022Review
- Automated Quantification and Network Analysis of Redox Dynamics in Neuronal Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multiparameter in vivo imaging in plants using genetically encoded fluorescent indicator multiplexing.Plant physiology · 2021Review
- Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration.Scientific reports · 2021Article
- Effects of Matrix pH on Spontaneous Transient Depolarization and Reactive Oxygen Species Production in Mitochondria.Frontiers in cell and developmental biology · 2021Article
- Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1.Channels (Austin, Tex.) · 2020Article
- In Vivo NADH/NADThe Plant cell · 2020Article
- Article
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses.Plant physiology · 2020Article
- Integrating Ultra-Weak Photon Emission Analysis in Mitochondrial Research.Frontiers in physiology · 2020Review
- Do angiosperms with highly divergent mitochondrial genomes have altered mitochondrial function?Mitochondrion · 2019Article
- Article
- Substrate-dependent and cyclophilin D-independent regulation of mitochondrial flashes in skeletal and cardiac muscle.Archives of biochemistry and biophysics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Mitochondrial ATP synthesis is driven by a membrane potential across the inner mitochondrial membrane; this potential is generated by the proton-pumping electron transport chain. A balance between proton pumping and dissipation of the proton gradient by ATP-synthase is critical to avoid formation of excessive reactive oxygen species due to overreduction of the electron transport chain. Here, we report a mechanism that regulates bioenergetic balance in individual mitochondria: a transient partial depolarization of the inner membrane. Single mitochondria in living Arabidopsis thaliana root cells undergo sporadic rapid cycles of partial dissipation and restoration of membrane potential, as observed by real-time monitoring of the fluorescence of the lipophilic cationic dye tetramethyl rhodamine methyl ester. Pulsing is induced in tissues challenged by high temperature, H(2)O(2), or cadmium. Pulses were coincident with a pronounced transient alkalinization of the matrix and are therefore not caused by uncoupling protein or by the opening of a nonspecific channel, which would lead to matrix acidification. Instead, a pulse is the result of Ca(2+) influx, which was observed coincident with pulsing; moreover, inhibitors of calcium transport reduced pulsing. We propose a role for pulsing as a transient uncoupling mechanism to counteract mitochondrial dysfunction and reactive oxygen species production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.