ArticleAnalytical and bioanalytical chemistry2012
De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics.
Article in Analytical and bioanalytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Developing Well-Annotated Species-Specific Protein Databases Using Comparative Proteogenomics: An Update.Advances in experimental medicine and biology · 2026Review
- Whole genome sequencing of neurotoxin-producingFrontiers in public health · 2025Article
- Validation of a clinical assay for botulinum neurotoxins through mass spectrometric detection.Journal of clinical microbiology · 2024Article
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- Recent Developments in Botulinum Neurotoxins Detection.Microorganisms · 2022Review
- Current Developments in Diagnostic Assays for Laboratory Confirmation and Investigation of Botulism.Journal of clinical microbiology · 2022Review
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- A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field.Proteomes · 2020Review
- Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B.Toxins · 2020Article
- Proposed BoNT/A and /B Peptide Substrates Cannot Detect Multiple Subtypes in the Endopep-MS Assay.Journal of analytical toxicology · 2020Article
- Molecular Characterization ofFoodborne pathogens and disease · 2019Article
- Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array technology.Scientific reports · 2019Article
- Variations in the Botulinum Neurotoxin Binding Domain and the Potential for Novel Therapeutics.Toxins · 2018Review
- Review
- Article
- Recommended Immunological Strategies to Screen for Botulinum Neurotoxin-Containing Samples.Toxins · 2015Article
- Article
- Review
- Isolation and functional characterization of the novel Clostridium botulinum neurotoxin A8 subtype.PloS one · 2015Article
- Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Botulinum neurotoxins (BoNTs) cause the disease botulism, which can be lethal if untreated. There are seven known serotypes of BoNT, A-G, defined by their response to antisera. Many serotypes are distinguished into differing subtypes based on amino acid sequence, and many subtypes are further differentiated into toxin variants. Previous work in our laboratory described the use of a proteomics approach to distinguish subtype BoNT/A1 from BoNT/A2 where BoNT identities were confirmed after searching data against a database containing protein sequences of all known BoNT/A subtypes. We now describe here a similar approach to differentiate subtypes BoNT/B1, /B2, /B3, /B4, and /B5. Additionally, to identify new subtypes or hitherto unpublished amino acid substitutions, we created an amino acid substitution database covering every possible amino acid change. We used this database to differentiate multiple toxin variants within subtypes of BoNT/B1 and B2. More importantly, with our amino acid substitution database, we were able to identify a novel BoNT/B subtype, designated here as BoNT/B7. These techniques allow for subtype and strain level identification of both known and unknown BoNT/B rapidly with no DNA required.
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