ArticleJournal of visualized experiments : JoVE2012
Reproducible mouse sciatic nerve crush and subsequent assessment of regeneration by whole mount muscle analysis.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 57 citations in OpenAlex.
- Lysophosphatidic acid mediates skeletal muscle fibrosis in denervation via activation of YAP/TAZ.JCI insight · 2026Article
- Recombinant Human Neuregulin1-β1 Significantly Reduces Schwannoma Growth in Mice.Annals of neurology · 2026Article
- Chronic alcohol drinking delays recovery from capsaicin- and nerve injury-induced hypersensitivity in mice.Alcohol (Fayetteville, N.Y.) · 2025Article
- Chronic alcohol drinking delays recovery from capsaicin- and nerve injury-induced hypersensitivity in mice.bioRxiv : the preprint server for biology · 2025Article
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- Impact of ivermectin on nerve regeneration following sciatic injury in mice: the consequences of dietary high fructose.Turkish journal of medical sciences · 2025Article
- Decoding the regulatory role of ATP synthase inhibitory factor 1 (ATPIF1) in Wallerian degeneration and peripheral nerve regeneration.Exploration (Beijing, China) · 2024Article
- Regeneration of neuromuscular synapses after acute and chronic denervation by inhibiting the gerozyme 15-prostaglandin dehydrogenase.Science translational medicine · 2023Article
- Base-Exchange Enabling the Visualization of SARM1 Activities in Sciatic Nerve-Injured Mice.ACS sensors · 2023Article
- Integrated analyses reveal evolutionarily conserved and specific injury response genes in dorsal root ganglion.Scientific data · 2022Article
- Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models.Bio-protocol · 2022Article
- Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis.Acta neuropathologica communications · 2022Article
- The macrophage: a key player in the pathophysiology of peripheral neuropathies.Journal of neuroinflammation · 2022Review
- Prevention of the foreign body response to implantable medical devices by inflammasome inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Axon Regeneration: A Subcellular Extension in Multiple Dimensions.Cold Spring Harbor perspectives in biology · 2022Review
- Blockage of neuromuscular glutamate receptors impairs reinnervation following nerve crush in adult mice.Frontiers in cellular neuroscience · 2022Article
- Involvement of the miR-363-5p/P2RX4 Axis in Regulating Schwann Cell Phenotype after Nerve Injury.International journal of molecular sciences · 2021Article
- Catfish Epidermal Preparation Accelerates Healing of Damaged Nerve in a Sciatic Nerve Crush Injury Rat Model.Frontiers in pharmacology · 2021Article
- Melatonin promotes regeneration of injured motor axons via MTJournal of pineal research · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Regeneration in the peripheral nervous system (PNS) is widely studied both for its relevance to human disease and to understand the robust regenerative response mounted by PNS neurons thereby possibly illuminating the failures of CNS regeneration(1). Sciatic nerve crush (axonotmesis) is one of the most common models of peripheral nerve injury in rodents(2). Crushing interrupts all axons but Schwann cell basal laminae are preserved so that regeneration is optimal(3,4). This allows the investigator to study precisely the ability of a growing axon to interact with both the Schwann cell and basal laminae(4). Rats have generally been the preferred animal models for experimental nerve crush. They are widely available and their lesioned sciatic nerve provides a reasonable approximation of human nerve lesions(5,4). Though smaller in size than rat nerve, the mouse nerve has many similar qualities. Most importantly though, mouse models are increasingly valuable because of the wide availability of transgenic lines now allows for a detailed dissection of the individual molecules critical for nerve regeneration(6, 7). Prior investigators have used multiple methods to produce a nerve crush or injury including simple angled forceps, chilled forceps, hemostatic forceps, vascular clamps, and investigator-designed clamps(8,9,10,11,12). Investigators have also used various methods of marking the injury site including suture, carbon particles and fluorescent beads(13,14,1). We describe our method to obtain a reproducibly complete sciatic nerve crush with accurate and persistent marking of the crush-site using a fine hemostatic forceps and subsequent carbon crush-site marking. As part of our description of the sciatic nerve crush procedure we have also included a relatively simple method of muscle whole mount we use to subsequently quantify regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.