ArticleIranian biomedical journal2011
Trans-differentiation of the adipose tissue-derived stem cells into neuron-like cells expressing neurotrophins by selegiline.
Article in Iranian biomedical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Functional assessment of local versus systemic adipose-derived stromal cell therapy in rodent peripheral nerve regeneration.Frontiers in neurology · 2025Article
- Single-Cell RNA-Seq Reveals the Pseudo-temporal Dynamic Evolution Characteristics of ADSCs to Neuronal Differentiation.Cellular and molecular neurobiology · 2024Article
- Directed Differentiation of Adipose-Derived Stem Cells Using Imprinted Cell-Like Topographies as a Growth Factor-Free Approach.Stem cell reviews and reports · 2024Review
- Progress and application of adipose-derived stem cells in the treatment of diabetes and its complications.Stem cell research & therapy · 2024Review
- Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research.International journal of molecular sciences · 2023Article
- Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells.International journal of stem cells · 2023Article
- Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke.Cell & bioscience · 2022Article
- Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl.Anatomy & cell biology · 2021Article
- Neuroprotective effects of selegiline on rat neural stem cells treated with hydrogen peroxide.Biomedical reports · 2018Article
- The regenerative role of adipose-derived stem cells (ADSC) in plastic and reconstructive surgery.International wound journal · 2017Review
- Advances in Adipose-Derived Stem Cells Isolation, Characterization, and Application in Regenerative Tissue Engineering.Stem cells international · 2016Review
- The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo.Cytotechnology · 2014Article
- Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor for repair of spinal cord contusion injury.Neural regeneration research · 2014Article
- Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage.In vitro cellular & developmental biology. Animal · 2014Article
- Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure.In vitro cellular & developmental biology. Animal · 2013Article
- Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats.Neural regeneration research · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdult stem cells (ASC) are undifferentiated cells found throughout the body. These cells are promising tools for cell replacement therapy in neurodegenerative disease. Adipose tissue is the most abundant and accessible source of ASC. This study was conducted to evaluate effect of selegiline on differentiation of adipose-derived stem cells (ADSC) into functional neuron-like cells (NLC), and also level of the neurotrophin expression in differentiated cells.
methodsADSC were transdifferentiated into NLC using selegiline where CD90, CD49d, CD31, CD106 and CD45 were used as markers for ADSC identification. Lipogenic and osteogenic differentiation of ADSC were used to characterize the ADSC. ADSC were treated with selegiline at different concentrations (from 10(-6) to 10(-11) mM) and time points (3, 6, 12, 24 and 48 h). Percentage of viable cells, nestin and neurofilament 68 (NF-68) immunoreactive cells were used as markers for differentiation. The optimal dose for neurotrophin expressions in differentiating cells was evaluated using reverse transcriptase-PCR. NLC function was evaluated by loading and unloading with FM1-43 dye.
resultsADSC were immunoreactive to CD90 (95.67 ± 2.26), CD49d (71.52 ± 6.64) and CD31 (0.6 ± 0.86), but no immunoreactivity was detected for CD106 and CD45. The results of neural differentiation showed the highest percentage of nestin and NF-68 positive cells at 10(-9) mM concentration of selegiline (exposed for 24 h). The differentiated cells expressed synapsin and neurotrophin genes except brain-derived neurotrophic factor.
conclusionADSC can be an alternative source in cell-based therapy for neurodegenerative diseases using selegiline to induce ADSC differentiation to neuronal lineage.
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