ArticlePloS one2012
Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Pharmaceutical Pollutants in Urban Rats Are Linked to Zoonotic Infection Risk.Environmental science & technology letters · 2026Article
- Serological and molecular epidemiology of leptospirosis and the role of dogs as sentinel for human infection in Nigeria.Heliyon · 2022Article
- Leptospira interrogans biofilm formation in Rattus norvegicus (Norway rats) natural reservoirs.PLoS neglected tropical diseases · 2021Article
- Correlation between renal distribution of leptospires during the acute phase and chronic renal dysfunction in a hamster model of infection with Leptospira interrogans.PLoS neglected tropical diseases · 2021Article
- A survey of zoonotic pathogens carried by house mouse and black rat populations in Yucatan, Mexico.Epidemiology and infection · 2017Article
- LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis.PLoS neglected tropical diseases · 2017Article
- Efficacy of the Rabbit Polyclonal Anti-leptospira Antibody against Homotype or Heterotype Leptospira Infection in Hamster.PLoS neglected tropical diseases · 2016Article
- Experimental Hamster Infection with a Strain of Leptospira borgpetersenii Ballum Isolated from a Reservoir Mouse in New Caledonia.The American journal of tropical medicine and hygiene · 2015Article
- A putative regulatory genetic locus modulates virulence in the pathogen Leptospira interrogans.Infection and immunity · 2014Article
- Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue.BMC veterinary research · 2014Article
- A prime-boost strategy using the novel vaccine candidate, LemA, protects hamsters against leptospirosis.Clinical and vaccine immunology : CVI · 2013Article
- Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM) for the detection and quantification of leptospires in kidney samples from the rat and hamster experimental models of leptospirosis. Using a virulent strain of Leptospira interrogans serovar Copenhageni, a chronic infection was established in the rat model, which were euthanized 28 days post-infection, while the hamster model simulated an acute infection and the hamsters were euthanized eight days after inoculation. Leptospires in the kidney samples were detected using culture isolation, qPCR and the IM, and quantified using qPCR and the IM. In both the acute and chronic infection models, the correlation between quantification by qPCR and the IM was found to be positive and statistically significant (P<0.05). Therefore, this study demonstrates that the IM is a viable alternative for not only the detection but also the quantification of leptospires, particularly when the use of qPCR is not feasible.
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