ArticlePloS one2012
Ex-vivo clonally expanded B lymphocytes infiltrating colorectal carcinoma are of mature immunophenotype and produce functional IgG.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Multimarker Assessment of B-Cell and Plasma Cell Subsets and Their Prognostic Role in the Colorectal Cancer Microenvironment.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025Article
- Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer.iScience · 2023Article
- B cells in pancreatic cancer stroma.World journal of gastroenterology · 2022Review
- Tumor-infiltrating lymphocytes for treatment of solid tumors: It takes two to tango?Frontiers in immunology · 2022Review
- Checkpoint molecules on infiltrating immune cells in colorectal tumor microenvironment.Frontiers in medicine · 2022Review
- Tumor-Infiltrating B Lymphocyte Profiling Identifies IgG-Biased, Clonally Expanded Prognostic Phenotypes in Triple-Negative Breast Cancer.Cancer research · 2021Article
- Long noncoding RNA: a dazzling dancer in tumor immune microenvironment.Journal of experimental & clinical cancer research : CR · 2020Review
- Immuno-Interface Score to Predict Outcome in Colorectal Cancer Independent of Microsatellite Instability Status.Cancers · 2020Article
- Patient-individual cancer cell lines and tissue analysis delivers no evidence of sequences from DNA viruses in colorectal cancer cells.BMC gastroenterology · 2020Article
- The Prognostic Importance of CD20Scientific reports · 2019Article
- Human Colorectal Carcinoma Infiltrating B Lymphocytes Are Active Secretors of the Immunoglobulin Isotypes A, G, and M.Cancers · 2019Article
- Deceleration of glycometabolism impedes IgG-producing B-cell-mediated tumor elimination by targeting SATB1.Immunology · 2019Article
- Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells.Frontiers in immunology · 2018Article
- Establishment and characterization of HROC69 - a Crohn´s related colonic carcinoma cell line and its matched patient-derived xenograft.Scientific reports · 2016Article
- Distribution and significance of interstitial fibrosis and stroma-infiltrating B cells in tongue squamous cell carcinoma.Oncology letters · 2016Article
- Tertiary Lymphoid Structure-Associated B Cells are Key Players in Anti-Tumor Immunity.Frontiers in immunology · 2015Review
- Capturing needles in haystacks: a comparison of B-cell receptor sequencing methods.BMC immunology · 2014Article
- Characterization of tumor-associated B-cell subsets in patients with colorectal cancer.Oncotarget · 2014Article
- Manipulation of tumour-infiltrating B cells and tertiary lymphoid structures: a novel anti-cancer treatment avenue?Cancer immunology, immunotherapy : CII · 2014Review
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6 authors.
Funding
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Abstract
backgroundTumor infiltrating B cells (TiBc) have not yet been investigated in detail. This may at least in part be due to technical difficulties. Here we describe a straightforward and reproducible method to isolate and culture TiBc from primary colorectal carcinomas (CRC). METHODS/
resultsTiBc cultures were generated by Epstein-Barr virus (EBV) immortalization. With this method, monoclonal TiBc cultures were obtained for 14/19 CRCs. As assessed by flow cytometry and ELISA, TiBc showed an activated immunophenotype (CD23(+), CD80(+)) and produced immunoglobulin (Ig; IgG secretion in 55% of the cultures). In functional in vitro analysis, most of the IgGs specifically bound to allogeneic CRC target cells. These data suggest that TiBc are antigen-experienced and thus may exhibit functionality in situ. Additionally, mini-cultures generated from 12 further CRCs revealed TiBc outgrowth exclusively in the presence of EBV.
conclusionIn summary, this simple method provides a cellular tool and our data set the stage for analysing the bivalent role of TiBc; being antigen-presenting cells on the one hand and tumor-specific antibody producers on the other. Additionally, the generation of long-term TiBc cultures and their monoclonal Ig may serve to identify novel tumor-specific antigens.
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