ArticlePloS one2012
Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Alphafuser: a parsimonious approach to predicting higher-order protein complexes.Acta crystallographica. Section D, Structural biology · 2026Article
- CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes.Structure (London, England : 1993) · 2025Article
- Current and future perspectives for structural biology at the Grenoble EPN campus: a comprehensive overview.Journal of synchrotron radiation · 2025Review
- ExploringRSC advances · 2024Article
- Bioactivity-Guided Synthesis: In Silico and In Vitro Studies ofMolecules (Basel, Switzerland) · 2023Article
- Prediction of chaperonin GroE substrates using small structural patterns of proteins.FEBS open bio · 2023Article
- Cytosolic phosphoglucose isomerase is essential for microsporogenesis and embryogenesis in Arabidopsis.Plant physiology · 2023Article
- ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.Journal of synchrotron radiation · 2022Article
- A structural basis for the functional differences between the cytosolic and plastid phosphoglucose isomerase isozymes.PloS one · 2022Article
- Synthetic Formatotrophs for One-Carbon Biorefinery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome.Faculty reviews · 2021Review
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- MADS transcription factors cooperate: complexities of complex formation.Journal of experimental botany · 2018Article
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- Multiple Forms of Glutamate Dehydrogenase in Animals: Structural Determinants and Physiological Implications.Biology · 2016Review
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- A novel binding of GTP stabilizes the structure and modulates the activities of human phosphoglucose isomerase/autocrine motility factor.Biochemistry and biophysics reports · 2015Article
- Evolution of aromatic β-glucoside utilization by successive mutational steps in Escherichia coli.Journal of bacteriology · 2015Article
- Crystallization of proteins from crude bovine rod outer segments.Methods in enzymology · 2015Article
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Authors and funding
11 authors.
Funding
Abstract
Structural biology and structural genomics projects routinely rely on recombinantly expressed proteins, but many proteins and complexes are difficult to obtain by this approach. We investigated native source proteins for high-throughput protein crystallography applications. The Escherichia coli proteome was fractionated, purified, crystallized, and structurally characterized. Macro-scale fermentation and fractionation were used to subdivide the soluble proteome into 408 unique fractions of which 295 fractions yielded crystals in microfluidic crystallization chips. Of the 295 crystals, 152 were selected for optimization, diffraction screening, and data collection. Twenty-three structures were determined, four of which were novel. This study demonstrates the utility of native source proteins for high-throughput crystallography.
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