ArticleMolecular & cellular proteomics : MCP2012
Plasma proteome dynamics: analysis of lipoproteins and acute phase response proteins with 2H2O metabolic labeling.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Rapid Antifungal Susceptibility Testing by Stimulated Raman Photothermal Imaging of DAnalytical chemistry · 2026Article
- Chronic Alcohol Consumption Reprograms Hepatic Metabolism Through Organelle-Specific Acetylation in Mice.Molecular & cellular proteomics : MCP · 2025Article
- A high-fat diet increases hepatic mitochondrial turnover through restricted acetylation in a NAFLD mouse model.American journal of physiology. Endocrinology and metabolism · 2023Article
- Metabolic labeling unveils alterations in the turnover of HDL-associated proteins during diabetes progression in mice.American journal of physiology. Endocrinology and metabolism · 2022Article
- Early Pro-Inflammatory Remodeling of HDL Proteome in a Model of Diet-Induced Obesity:International journal of molecular sciences · 2020Article
- Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes.Biomolecules · 2020Article
- Calculation of the Protein Turnover Rate Using the Number of IncorporatedAnalytical chemistry · 2019Article
- HDL flux is higher in patients with nonalcoholic fatty liver disease.American journal of physiology. Endocrinology and metabolism · 2019Article
- Hepatic Mitochondrial Defects in a Nonalcoholic Fatty Liver Disease Mouse Model Are Associated with Increased Degradation of Oxidative Phosphorylation Subunits.Molecular & cellular proteomics : MCP · 2018Article
- Poisson Model To Generate Isotope Distribution for Biomolecules.Journal of proteome research · 2018Article
- Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism.Mass spectrometry reviews · 2018Review
- Increased serotransferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes.Free radical biology & medicine · 2017Article
- Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure.Clinical epigenetics · 2017Article
- Monitoring the synthesis of biomolecules using mass spectrometry.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Proteome Dynamics Reveals Pro-Inflammatory Remodeling of Plasma Proteome in a Mouse Model of NAFLD.Journal of proteome research · 2016Article
- A Western diet induced NAFLD in LDLR(-/)(-) mice is associated with reduced hepatic glutathione synthesis.Free radical biology & medicine · 2016Article
- Article
- Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics.Journal of diabetes research · 2016Article
- Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.Journal of proteome research · 2015Article
- Mitochondrial protein turnover: methods to measure turnover rates on a large scale.Journal of molecular and cellular cardiology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Understanding the pathologies related to the regulation of protein metabolism requires methods for studying the kinetics of individual proteins. We developed a (2)H(2)O metabolic labeling technique and software for protein kinetic studies in free living organisms. This approach for proteome dynamic studies requires the measurement of total body water enrichments by GC-MS, isotopic distribution of the tryptic peptide by LC-MS/MS, and estimation of the asymptotical number of deuterium incorporated into a peptide by software. We applied this technique to measure the synthesis rates of several plasma lipoproteins and acute phase response proteins in rats. Samples were collected at different time points, and proteins were separated by a gradient gel electrophoresis. (2)H labeling of tryptic peptides was analyzed by ion trap tandem mass spectrometry (LTQ MS/MS) for measurement of the fractional synthesis rates of plasma proteins. The high sensitivity of LTQ MS in zoom scan mode in combination with (2)H label amplification in proteolytic peptides allows detection of the changes in plasma protein synthesis related to animal nutritional status. Our results demonstrate that fasting has divergent effects on the rate of synthesis of plasma proteins, increasing synthesis of ApoB 100 but decreasing formation of albumin and fibrinogen. We conclude that this technique can effectively measure the synthesis of plasma proteins and can be used to study the regulation of protein homeostasis under physiological and pathological conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.