ArticleJournal of cell science2012
Spatiotemporal resolution of mast cell granule exocytosis reveals correlation with Ca2+ wave initiation.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Mast cell granule motility and exocytosis is driven by dynamic microtubule formation and kinesin-1 motor function.PloS one · 2022Article
- MYO1F Regulates IgE and MRGPRX2-Dependent Mast Cell Exocytosis.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics.Journal of cell science · 2021Article
- Tetraspanins in the regulation of mast cell function.Medical microbiology and immunology · 2020Review
- Optical Tracking of Nanometer-Scale Cellular Membrane Deformation Associated with Single Vesicle Release.ACS sensors · 2019Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- High-throughput screening system for dynamic monitoring of exocytotic vesicle trafficking in mast cells.PloS one · 2018Article
- Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis.Scientific reports · 2017Article
- Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis.Biology open · 2017Article
- Munc13-4 functions as a CaMolecular biology of the cell · 2017Article
- Nanoimaging granule dynamics and subcellular structures in activated mast cells using soft X-ray tomography.Scientific reports · 2016Article
- Different activation signals induce distinct mast cell degranulation strategies.The Journal of clinical investigation · 2016Article
- Roles for Ca2+ mobilization and its regulation in mast cell functions: recent progress.Biochemical Society transactions · 2016Review
- Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc.Micromachines · 2016Article
- Non-Faradaic Electrochemical Detection of Exocytosis from Mast and Chromaffin Cells Using Floating-Gate MOS Transistors.Scientific reports · 2015Article
- Real-time imaging of Ca(2+) mobilization and degranulation in mast cells.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Rictor negatively regulates high-affinity receptors for IgE-induced mast cell degranulation.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Characterization of mast cell secretory granules and their cell biology.DNA and cell biology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mast cell activation initiated by antigen-mediated crosslinking of IgE receptors results in stimulated exocytosis of secretory lysosomes in the process known as degranulation. Much has been learned about the molecular mechanisms important for this process, including the crucial role of Ca(2+) mobilization, but spatio-temporal relationships between stimulated Ca(2+) mobilization and granule exocytosis are incompletely understood. Here we use a novel imaging-based method that uses fluorescein isothiocyanate (FITC)-dextran as a reporter for granule exocytosis in RBL mast cells and takes advantage of the pH sensitivity of FITC. We demonstrate the selectivity of FITC-dextran, accumulated by fluid-phase uptake, as a marker for secretory lysosomes, and we characterize its capacity to delineate different exocytotic events, including full fusion, kiss-and-run transient fusion and compound exocytosis. Using this method, we find strong dependence of degranulation kinetics on the duration of cell to substrate attachment. We combine imaging of degranulation and Ca(2+) dynamics to demonstrate a spatial relationship between the sites of Ca(2+) wave initiation in extended cell protrusions and exocytosis under conditions of limited antigen stimulation. In addition, we find that the spatially proximal Ca(2+) signaling and secretory events correlate with participation of TRPC1 channels in Ca(2+) mobilization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.