ArticleThe Journal of biological chemistry2012
Imaging P2X4 receptor lateral mobility in microglia: regulation by calcium and p38 MAPK.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- TSC-associated microglial hyperactivity: enhanced calcium signaling, metabolism, and phagocytosis.Acta neuropathologica · 2026Article
- Comparison of Microglial Morphology and Function in Primary Cerebellar Cell Cultures on Collagen and Collagen-Mimetic Hydrogels.Biomedicines · 2022Article
- Purinoreceptors and ectonucleotidases control ATP-induced calcium waveforms and calcium-dependent responses in microglia: Roles of P2 receptors and CD39 in ATP-stimulated microglia.Frontiers in physiology · 2022Article
- Mathematical modelling of human P2X-mediated plasma membrane electrophysiology and calcium dynamics in microglia.PLoS computational biology · 2021Article
- Effects of Metformin on Spontaneous CaInternational journal of molecular sciences · 2021Article
- Review
- Optogenetic activation of spinal microglia triggers chronic pain in mice.PLoS biology · 2021Article
- Article
- Simulation of P2X-mediated calcium signalling in microglia.The Journal of physiology · 2019Article
- Preclinical evaluation of avermectins as novel therapeutic agents for alcohol use disorders.Psychopharmacology · 2018Review
- Ethanol differentially modulates P2X4 and P2X7 receptor activity and function in BV2 microglial cells.Neuropharmacology · 2018Article
- Modulation of Central Synapses by Astrocyte-Released ATP and Postsynaptic P2X Receptors.Neural plasticity · 2017Review
- P2X4 Receptor Function in the Nervous System and Current Breakthroughs in Pharmacology.Frontiers in pharmacology · 2017Review
- P2X4 Receptor Reporter Mice: Sparse Brain Expression and Feeding-Related Presynaptic Facilitation in the Arcuate Nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Polymorphisms in CAMKK2 may predict sensory neuropathy in African HIV patients.Journal of neurovirology · 2016Article
- Microglia-derived purines modulate mossy fibre synaptic transmission and plasticity through P2X4 and A1 receptors.The European journal of neuroscience · 2016Article
- Microglia in the spinal cord and neuropathic pain.Journal of diabetes investigation · 2016 · on this mapReview
- Single-quantum-dot tracking reveals altered membrane dynamics of an attention-deficit/hyperactivity-disorder-derived dopamine transporter coding variant.ACS chemical neuroscience · 2015Article
- Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission.Nature neuroscience · 2015Article
- The ATP Receptors P2X7 and P2X4 Modulate High Glucose and Palmitate-Induced Inflammatory Responses in Endothelial Cells.PloS one · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
ATP-gated ionotropic P2X4 receptors are up-regulated in activated microglia and are critical for the development of neuropathic pain, a microglia-associated disorder. However, the nature of how plasma membrane P2X4 receptors are regulated in microglia is not fully understood. We used single-molecule imaging to track quantum dot-labeled P2X4 receptors to explore P2X4 receptor mobility in the processes of resting and activated microglia. We find that plasma membrane P2X4 receptor lateral mobility in resting microglial processes is largely random, consisting of mobile and slowly mobile receptors. Moreover, lateral mobility is P2X subunit- and cell-specific, increased in an ATP activation and calcium-dependent manner, and enhanced in activated microglia by the p38 MAPK pathway that selectively regulates slowly mobile receptors. Thus, our data indicate that P2X4 receptors are dynamically regulated mobile ATP sensors, sampling more of the plasma membrane in response to ATP and during the activated state of microglia that is associated with nervous system dysfunction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.