ArticleThe Journal of biological chemistry2012
Impact of oxidative stress on ascorbate biosynthesis in Chlamydomonas via regulation of the VTC2 gene encoding a GDP-L-galactose phosphorylase.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 107 citations in OpenAlex.
- Horizontal gene transfer and diploidy illuminate evolution and stress adaptation in oleaginous Scenedesmaceae (Chlorophyta).Nature communications · 2026Article
- Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.Journal of experimental botany · 2025Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Genome-wide association study unveils ascorbate regulation by PAS/LOV PROTEIN during high light acclimation.Plant physiology · 2023Article
- Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell.Communications biology · 2023Article
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Chlamydomonas as a model for reactive oxygen species signaling and thiol redox regulation in the green lineage.Plant physiology · 2021Review
- Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048.Extremophiles : life under extreme conditions · 2021Article
- Diverse Biosynthetic Pathways and Protective Functions against Environmental Stress of Antioxidants in Microalgae.Plants (Basel, Switzerland) · 2021Review
- Co-expression networks in Chlamydomonas reveal significant rhythmicity in batch cultures and empower gene function discovery.The Plant cell · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection.Nature communications · 2021Article
- Molecular Mechanisms Underlying the Acclimation ofFrontiers in plant science · 2021Article
- Article
- Ascorbate peroxidase 4 plays a role in the tolerance of Chlamydomonas reinhardtii to photo-oxidative stress.Scientific reports · 2020Article
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Article
- The Effect of Light in Vitamin C Metabolism Regulation and Accumulation in Mung Bean (Vigna radiata) Germination.Plant foods for human nutrition (Dordrecht, Netherlands) · 2020Article
- Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses.Plants (Basel, Switzerland) · 2020Review
- Ascorbate Deficiency Does Not Limit Nonphotochemical Quenching inPlant physiology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
The L-galactose (Smirnoff-Wheeler) pathway represents the major route to L-ascorbic acid (vitamin C) biosynthesis in higher plants. Arabidopsis thaliana VTC2 and its paralogue VTC5 function as GDP-L-galactose phosphorylases converting GDP-L-galactose to L-galactose-1-P, thus catalyzing the first committed step in the biosynthesis of L-ascorbate. Here we report that the L-galactose pathway of ascorbate biosynthesis described in higher plants is conserved in green algae. The Chlamydomonas reinhardtii genome encodes all the enzymes required for vitamin C biosynthesis via the L-galactose pathway. We have characterized recombinant C. reinhardtii VTC2 as an active GDP-L-galactose phosphorylase. C. reinhardtii cells exposed to oxidative stress show increased VTC2 mRNA and L-ascorbate levels. Genes encoding enzymatic components of the ascorbate-glutathione system (e.g. ascorbate peroxidase, manganese superoxide dismutase, and dehydroascorbate reductase) are also up-regulated in response to increased oxidative stress. These results indicate that C. reinhardtii VTC2, like its plant homologs, is a highly regulated enzyme in ascorbate biosynthesis in green algae and that, together with the ascorbate recycling system, the L-galactose pathway represents the major route for providing protective levels of ascorbate in oxidatively stressed algal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.