ArticleThe Journal of biological chemistry2012
IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Drug-resistantClinical microbiology reviews · 2025Pooled it
- Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species.Applied microbiology and biotechnology · 2026Article
- Biophysical characterization of Eag chaperones suggests the mechanism of effector transmembrane domain release.Nature communications · 2026Article
- Article
- Transcriptome profiling of type VI secretion system core geneMicrobiology spectrum · 2024Article
- The role of type VI secretion system genes in antibiotic resistance and virulence inFrontiers in cellular and infection microbiology · 2024Article
- Protein-Protein Interactions: Co-immunoprecipitation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Parallel loss of type VI secretion systems in two multi-drug-resistantMicrobial genomics · 2023Article
- Virulence genes identification inVeterinary world · 2023Article
- Type VI secretion system (T6SS) inIranian journal of microbiology · 2023Article
- Structure and Function of the Dot/Icm T4SS.bioRxiv : the preprint server for biology · 2023Article
- The type-VI secretion system of the beneficial symbiontMicrobiology (Reading, England) · 2023Review
- Uncovering the Secretion Systems ofAntibiotics (Basel, Switzerland) · 2023Review
- Article
- Subcellular localization of Type VI secretion system assembly in response to cell-cell contact.The EMBO journal · 2022Article
- Virulence Genotype and Correlation of Clinical Severeness with Presence of the Type VI Secretion System inInfection and drug resistance · 2022Article
- Potential dsRNAs can be delivered to aquatic for defense pathogens.Frontiers in bioengineering and biotechnology · 2022Review
- Activation of the Type VI Secretion System in the Squid SymbiontJournal of bacteriology · 2021Article
- Vibrio vulnificus induces the death of a major bacterial species in the mouse gut via cyclo-Phe-Pro.Microbiome · 2021Article
- The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly inmBio · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The type VI secretion system (T6SS) with diversified functions is widely distributed in pathogenic Proteobacteria. The IcmF (intracellular multiplication protein F) family protein TssM is a conserved T6SS inner membrane protein. Despite the conservation of its Walker A nucleotide-binding motif, the NTPase activity of TssM and its role in T6SS remain obscure. In this study, we characterized TssM in the plant pathogen Agrobacterium tumefaciens and provided the first biochemical evidence for TssM exhibiting ATPase activity to power the secretion of the T6SS hallmark protein, hemolysin-coregulated protein (Hcp). Amino acid substitutions in the Walker A motif of TssM caused reduced ATP binding and hydrolysis activity. Importantly, we discovered the Walker B motif of TssM and demonstrated that it is critical for ATP hydrolysis activity. Protein-protein interaction studies and protease susceptibility assays indicated that TssM undergoes an ATP binding-induced conformational change and that subsequent ATP hydrolysis is crucial for recruiting Hcp to interact with the periplasmic domain of the TssM-interacting protein TssL (an IcmH/DotU family protein) into a ternary complex and mediating Hcp secretion. Our findings strongly argue that TssM functions as a T6SS energizer to recruit Hcp into the TssM-TssL inner membrane complex prior to Hcp secretion across the outer membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.