ArticleProceedings of the National Academy of Sciences of the United States of America2012
Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers, 1 of them a synthesis that pooled it.
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93 citing papers in PubMed, 1 synthesis or guideline pooled it, 299 citations in OpenAlex.
- Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite.Plant physiology · 2014Pooled it
- Genomic analysis of population structure and target-site mutations of Tetranychus truncatus in China.Pest management science · 2026Article
- Disruption of chitin synthases impairs tick feeding and reproduction, validating a broad-spectrum acaricide target.Frontiers in cellular and infection microbiology · 2026Article
- Bulk Segregant Analysis Reveals Genomic Regions Associated With Imidacloprid Resistance in the Colorado Potato Beetle.Ecology and evolution · 2025Article
- The evolutionary dilemma of broad-spectrum insecticides.Bioscience · 2025Review
- Recurrent mutations drive the rapid evolution of pesticide resistance in the two-spotted spider miteeLife · 2025Article
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- Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies.Scientific data · 2024Article
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- Integration of metabolomics and transcriptomics reveals the regulation mechanism of the phenylpropanoid biosynthesis pathway in insect resistance traits inHorticulture research · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- A nuclear receptor HR96-related gene underlies large trans-driven differences in detoxification gene expression in a generalist herbivore.Nature communications · 2023Article
- Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest.Evolutionary applications · 2023Article
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- Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype.Insects · 2022Article
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- Utility and challenges of using whole-genome resequencing to detect emerging insect and mite resistance in agroecosystems.Evolutionary applications · 2022Review
- Review
33 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 5 institutions in 5 countries.
Funding
Abstract
Because of its importance to the arthropod exoskeleton, chitin biogenesis is an attractive target for pest control. This point is demonstrated by the economically important benzoylurea compounds that are in wide use as highly specific agents to control insect populations. Nevertheless, the target sites of compounds that inhibit chitin biogenesis have remained elusive, likely preventing the full exploitation of the underlying mode of action in pest management. Here, we show that the acaricide etoxazole inhibits chitin biogenesis in Tetranychus urticae (the two-spotted spider mite), an economically important pest. We then developed a population-level bulk segregant mapping method, based on high-throughput genome sequencing, to identify a locus for monogenic, recessive resistance to etoxazole in a field-collected population. As supported by additional genetic studies, including sequencing across multiple resistant strains and genetic complementation tests, we associated a nonsynonymous mutation in the major T. urticae chitin synthase (CHS1) with resistance. The change is in a C-terminal transmembrane domain of CHS1 in a highly conserved region that may serve a noncatalytic but essential function. Our finding of a target-site resistance mutation in CHS1 shows that at least one highly specific chitin biosynthesis inhibitor acts directly to inhibit chitin synthase. Our work also raises the possibility that other chitin biogenesis inhibitors, such as the benzoylurea compounds, may also act by inhibition of chitin synthases. More generally, our genetic mapping approach should be powerful for high-resolution mapping of simple traits (resistance or otherwise) in arthropods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.