ArticleProceedings of the National Academy of Sciences of the United States of America2012
Stoichiometry of proton translocation by respiratory complex I and its mechanistic implications.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 107 citations in OpenAlex.
- Enzymatic oxygen reduction dominates overpotential-driven thermogenesis in mitochondria.Chemical science · 2026Article
- Direct dapagliflozin exposure enhances respiration and membrane hyperpolarization in isolated cardiac mitochondria.Frontiers in physiology · 2026Article
- Decoupled respiration in electro-active bacteria.Communications biology · 2025Article
- Targeting the Electron Transport System for Enhanced Longevity.Biomolecules · 2025Review
- Metagenomic Insights into the Abundance of Iron-Reducing Microorganisms in a Petroleum-Contaminated Iron-Rich Aquifer.Microorganisms · 2025Article
- Identification of complex III, NQR, and SDH as primary bioenergetic enzymes during the stationary phase ofFrontiers in microbiology · 2024Article
- Evidential deep learning for trustworthy prediction of enzyme commission number.Briefings in bioinformatics · 2023Article
- Mitochondrial DNA mutations in ageing and cancer.Molecular oncology · 2022Review
- Potential metabolic mechanisms for inhibited chloroplast nitrogen assimilation under high CO2.Plant physiology · 2021Article
- Metabolic energy conservation for fermentative product formation.Microbial biotechnology · 2021Review
- Architecture of bacterial respiratory chains.Nature reviews. Microbiology · 2021Review
- The Redox-Active Tyrosine Is Essential for Proton Pumping in Cytochrome c Oxidase.Frontiers in chemistry · 2021Article
- Thermodynamic efficiency, reversibility, and degree of coupling in energy conservation by the mitochondrial respiratory chain.Communications biology · 2020Article
- Energy-converting hydrogenases: the link between HCellular and molecular life sciences : CMLS · 2020Review
- Mitochondrial electron transport chain, ROS generation and uncoupling (Review).International journal of molecular medicine · 2019Article
- An update of the chemiosmotic theory as suggested by possible proton currents inside the coupling membrane.Open biology · 2019Review
- How cardiolipin modulates the dynamics of respiratory complex I.Science advances · 2019Article
- Redox-coupled quinone dynamics in the respiratory complex I.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- How inter-subunit contacts in the membrane domain of complex I affect proton transfer energetics.Biochimica et biophysica acta. Bioenergetics · 2018Article
- Long-range proton-coupled electron transfer in biological energy conversion: towards mechanistic understanding of respiratory complex I.Journal of the Royal Society, Interface · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Complex I (NADH-ubiquinone oxidoreductase) in the respiratory chain of mitochondria and several bacteria functions as a redox-driven proton pump that contributes to the generation of the protonmotive force across the inner mitochondrial or bacterial membrane and thus to the aerobic synthesis of ATP. The stoichiometry of proton translocation is thought to be 4 H(+) per NADH oxidized (2 e(-)). Here we show that a H(+)/2 e(-) ratio of 3 appears more likely on the basis of the recently determined H(+)/ATP ratio of the mitochondrial F(1)F(o)-ATP synthase of animal mitochondria and of a set of carefully determined ATP/2 e(-) ratios for different segments of the mitochondrial respiratory chain. This lower H(+)/2 e(-) ratio of 3 is independently supported by thermodynamic analyses of experiments with both mitochondria and submitochondrial particles. A reduced H(+)/2 e(-) stoichiometry of 3 has important mechanistic implications for this proton pump. In a rough mechanistic model, we suggest a concerted proton translocation mechanism in the three homologous and tightly packed antiporter-like subunits L, M, and N of the proton-translocating membrane domain of complex I.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.