ArticleLab on a chip2012
Efficient on-chip isolation of HIV subtypes.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy.APL bioengineering · 2026Article
- Spatiotemporal Confinements of Distance-Dependent Emitters for Enhancing Plasmonic Signals.ACS applied materials & interfaces · 2026Article
- Spatial Designs on Metamaterial Sensors for Enhancing Signals and Detecting Extracellular Vesicles.ACS applied materials & interfaces · 2025Article
- Surface Functionalization of ITO for Dual-Mode Hypoxia-Associated Cancer Biomarker Detection.Biosensors · 2025Article
- Article
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- The Role of Ligand Rebinding and Facilitated Dissociation on the Characterization of Dissociation Rates by Surface Plasmon Resonance (SPR) and Benchmarking Performance Metrics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- A Novel Microfluidic Chip for Fast, Sensitive Quantification of Plasma Extracellular Vesicles as Biomarkers in Patients With Osteosarcoma.Frontiers in oncology · 2021Article
- Microfluidic Isolation and Enrichment of Nanoparticles.ACS nano · 2020Review
- Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection.Viruses · 2020Review
- Article
- Micropatterned macroporous structures in microfluidic devices for viral separation from whole blood.The Analyst · 2017Article
- An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer.Scientific reports · 2017Article
- Photonic crystals: emerging biosensors and their promise for point-of-care applications.Chemical Society reviews · 2017Review
- Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection.International journal of nanomedicine · 2017Article
- Microfluidic devices with templated regular macroporous structures for HIV viral capture.The Analyst · 2016Article
- Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications.Scientific reports · 2016Article
- Advances in addressing technical challenges of point-of-care diagnostics in resource-limited settings.Expert review of molecular diagnostics · 2016Review
- Micro- and nanodevices integrated with biomolecular probes.Biotechnology advances · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
HIV has caused a global pandemic over the last three decades. There is an unmet need to develop point-of-care (POC) viral load diagnostics to initiate and monitor antiretroviral treatment in resource-constrained settings. Particularly, geographical distribution of HIV subtypes poses significant challenges for POC immunoassays. Here, we demonstrated a microfluidic device that can effectively capture various subtypes of HIV particles through anti-gp120 antibodies, which were immobilized on the microchannel surface. We first optimized an antibody immobilization process using fluorescent antibodies, quantum dot staining and AFM studies. The results showed that anti-gp120 antibodies were immobilized on the microchannel surface with an elevated antibody density and uniform antibody orientation using a Protein G-based surface chemistry. Further, RT-qPCR analysis showed that HIV particles of subtypes A, B and C were captured repeatably with high efficiencies of 77.2 ± 13.2%, 82.1 ± 18.8, and 80.9 ± 14.0% from culture supernatant, and 73.2 ± 13.6, 74.4 ± 14.6 and 78.3 ± 13.3% from spiked whole blood at a viral load of 1000 copies per mL, respectively. HIV particles of subtypes A, B and C were captured with high efficiencies of 81.8 ± 9.4%, 72.5 ± 18.7, and 87.8 ± 3.2% from culture supernatant, and 74.6 ± 12.9, 75.5 ± 6.7 and 69.7 ± 9.5% from spiked whole blood at a viral load of 10,000 copies per mL, respectively. The presented immuno-sensing device enables the development of POC on-chip technologies to monitor viral load and guide antiretroviral treatment (ART) in resource-constrained settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.