ArticlePlanta2012
A mutation in the E2 subunit of the mitochondrial pyruvate dehydrogenase complex in Arabidopsis reduces plant organ size and enhances the accumulation of amino acids and intermediate products of the TCA cycle.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Characterizing seed dormancy in Epimedium brevicornu Maxim.: Development of novel chill models and determination of dormancy release mechanisms by transcriptomics.BMC plant biology · 2024Article
- Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response.Science advances · 2024Article
- DIA-Based Quantitative Proteomics in the Flower Buds of TwoInternational journal of molecular sciences · 2024Article
- TCA cycle impairment leads to PIN2 internalization and degradation via reduced MAB4 level and ARA6 components in Arabidopsis roots.Frontiers in plant science · 2024Article
- Functional Genome Analyses Reveal the Molecular Basis of Oil Accumulation in Developing Seeds of Castor Beans.International journal of molecular sciences · 2023Article
- γ-Aminobutyric Acid Priming Alleviates Acid-Aluminum Toxicity to Creeping Bentgrass by Regulating Metabolic Homeostasis.International journal of molecular sciences · 2023Article
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Identification of Genetic Variations and Candidate Genes Responsible for Stalk Sugar Content and Agronomic Traits in Fresh Corn via GWAS across Multiple Environments.International journal of molecular sciences · 2022Article
- Physiological and protein profiling analysis provides insight into the underlying molecular mechanism of potato tuber development regulated by jasmonic acid in vitro.BMC plant biology · 2022Article
- The versatility of plant organic acid metabolism in leaves is underpinned by mitochondrial malate-citrate exchange.The Plant cell · 2021Article
- Nitrate alleviates ammonium toxicity in wheat (Plant signaling & behavior · 2021Article
- The mitochondrial pyruvate carrier (MPC) complex mediates one of three pyruvate-supplying pathways that sustain Arabidopsis respiratory metabolism.The Plant cell · 2021Article
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria.Plant physiology · 2021Article
- Decreased Vascular Bundle 1 affects mitochondrial and plant development in rice.Rice (New York, N.Y.) · 2021Article
- iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings.PeerJ · 2021Article
- Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development.Plant physiology · 2020Article
- Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana.Journal of experimental botany · 2019Article
- Mitochondrial Pyruvate Dehydrogenase Contributes to Auxin-Regulated Organ Development.Plant physiology · 2019Article
- Mitochondrial pyruvate carrier 1 mediates abscisic acid-regulated stomatal closure and the drought response by affecting cellular pyruvate content in Arabidopsis thaliana.BMC plant biology · 2017Article
- Metabolite profiling and associated gene expression reveal two metabolic shifts during the seed-to-seedling transition in Arabidopsis thaliana.Plant molecular biology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrial pyruvate dehydrogenase complex (mtPDC) plays a pivotal role in controlling the entry of carbon into the tricarboxylic acid (TCA) cycle for energy production. This multi-enzyme complex consists of three components: E1, E2, and E3. In Arabidopsis, there are three genes, mtE2-1, mtE2-2, and mtE2-3, which encode the putative mtPDC E2 subunit but how each of them contributes to the total mtPDC activity remains unknown. In this work, we characterized an Arabidopsis mutant, m132, that has abnormal small organs. Molecular cloning indicated that the phenotype of m132 is caused by a mutation in the mtE2-1 gene, which results in a truncation of 109 amino acids at the C-terminus of the encoded protein. In m132, mtPDC activity is only 30% of the WT and ATP production is severely impaired. The mutation in the mtE2-1 gene also leads to the over-accumulation of most intermediate products of the TCA cycle and of all the amino acids for protein synthesis. Our results suggest that, among the three mtE2 genes, mtE2-1 is a major contributor to the function of Arabidopsis mtPDC and that the functional disruption of mtE2-1 profoundly affects plant growth and development, as well as its metabolism.
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