ArticlePediatric research2012
Androgen receptor is overexpressed in boys with severe hypospadias, and ZEB1 regulates androgen receptor expression in human foreskin cells.
Article in Pediatric research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Unraveling the Androgen Receptor's Role in Hypospadias: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2026Pooled it
- Isolation and characterization of epithelial cells and fibroblasts from the human penile urethra.Frontiers in bioengineering and biotechnology · 2025Article
- Downregulated androgen receptor expression in preputial tissues of children with hypospadias.Frontiers in pediatrics · 2025Article
- AR loss in prostate cancer stroma mediated by NF-κB and p38-MAPK signaling disrupts stromal morphogen production.Oncogene · 2024Article
- Quantitative proteomics reveals specific protein regulation of severe hypospadias.Translational andrology and urology · 2022Article
- Dissecting the effects of androgen deprivation therapy on cadherin switching in advanced prostate cancer: A molecular perspective.Oncology research · 2022Review
- New frontiers on the molecular underpinnings of hypospadias according to severity.Arab journal of urology · 2020Review
- A Proteomics Signature of Mild Hypospadias: A Pilot Study.Frontiers in pediatrics · 2020Article
- Severity-Dependent Profile of the Metabolome in Hypospadias.Frontiers in pediatrics · 2020Article
- Langerhans cells in hypospadias: an analysis of Langerin (CD207) and HLA-DR on epidermal sheets and full thickness skin sections.BMC urology · 2019Article
- Smoking during pregnancy increases chemerin expression in neonatal tissue.Experimental physiology · 2019Article
- Increased birth weight is associated with altered gene expression in neonatal foreskin.Journal of developmental origins of health and disease · 2017Article
- Article
- Androgen Receptor Expression in Hypospadias.Journal of Indian Association of Pediatric SurgeonsArticle
- Immunohistochemical expression analysis of the human fetal lower urogenital tract.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
introductionZEB1 is overexpressed in patients with severe hypospadias. We examined the interaction between ZEB1 and the androgen receptor (AR) in vitro and the expression of AR in boys with hypospadias.
resultsZEB1 and AR colocalize to the nucleus. Estrogen upregulated ZEB1 and AR expression. Chromatin immunoprecipitation (ChIP) demonstrated that ZEB1 binds to an E-box sequence in the AR gene promoter. AR expression is higher in subjects with severe hypospadias than those with mild hypospadias and control subjects (P < 0.05). ZEB1 physically interacts with AR in human foreskin cells. DISCUSSION: AR is overexpressed in patients with severe hypospadias. Environmental estrogenic compounds may increase the risk of hypospadias by facilitating the interaction between ZEB1 and AR.
methodsHs68 cells, a fibroblast cell line derived from neonatal human foreskin, were exposed to 0, 10, and 100 nmol/l of estrogen, after which the cellular localization of ZEB1 and AR was assessed using immunocytochemistry. To determine if ZEB1 interacted with the AR gene, ChIP was performed using ZEB1 antibody and polymerase chain reaction (PCR) for AR. Second, AR expression was quantified using real-time PCR and western blot in normal subjects (n = 32), and subjects with mild (n = 16) and severe hypospadia (n = 16).
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