ArticleVirology journal2012
Molecular characterization of a novel mycovirus in the cultivated mushroom, Lentinula edodes.
Article in Virology journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Deep sequencing of single Pleurotus ostreatus strain revealed mixed infection of seven RNA viruses.BMC genomics · 2026Article
- High-Throughput Sequencing-Guided Development and Validation of an RT-dPCR Assay for Sensitive Detection of Lentinula edodes Spherical Virus in Shiitake Mushrooms.Current microbiology · 2026Article
- New insights into temperature-impacted mycovirus-fungus interactions regulated by a microRNA inJournal of virology · 2025Article
- Discovery of novel mycoviruses from fungi associated with mango leaf spots.Frontiers in microbiology · 2025Article
- Analysis of Leishbuviridae from Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2025Article
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- Seven new mycoviruses identified from isolated ascomycetous macrofungi.Virus research · 2024Article
- Exploring the Mycovirus Universe: Identification, Diversity, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- Identification of novel RNA mycoviruses from wild mushroom isolates in Japan.Virus research · 2023Article
- Diversity of mycoviruses in edible fungi.Virus genes · 2022Review
- The Mycovirome in a Worldwide Collection of the Brown Rot FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Characterization of a Novel Mitovirus InfectingFrontiers in microbiology · 2021Article
- Molecular Characterization of a Novel Polymycovirus FromFrontiers in microbiology · 2020Article
- Metagenomes of a Freshwater Charavirus from British Columbia Provide a Window into Ancient Lineages of Viruses.Viruses · 2019Article
- Viruses infecting macrofungi.Virusdisease · 2018Review
- Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible MushroomMycobiology · 2016Article
- Hypovirulence ofFrontiers in microbiology · 2016Article
- A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Occurrence of dsRNA Mycovirus (LeV-FMRI0339) in the Edible Mushroom Lentinula edodes and Meiotic Stability of LeV-FMRI0339 among Monokaryotic Progeny.The plant pathology journal · 2013Article
- Isolation and characterization of a mycovirus in Lentinula edodes.Journal of microbiology (Seoul, Korea) · 2013Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the 1970s, mycoviruses were identified that infected the edible mushroom Lentinula edodes (shiitake), but they were not regarded as causal agents for mushroom diseases. None of their genes has been sequenced. In this study, the dsRNA genome of a mycovirus recently found in a shiitake commercial strain was sequenced and its molecular structure was characterized.
methodsA cDNA library was constructed from a dsRNA purified from the fruiting body of L. edodes. The virus was tentatively named L. edodes mycovirus HKB (LeV). Based on the deduced RNA-dependent RNA polymerase (RdRp) sequence, phylogenetic analysis of LeV was conducted. Because no virion particles associated with the dsRNA were observed by electron microscopic observation, atomic force microscopy (AFM) observation was chosen for achieving molecular imaging of the virus.
resultsThe 11,282-bp genome of LeV was obtained. The genome encoded two open reading frames (ORFs). ORF1 coded for a hypothetical protein and ORF2 for a putative RdRp, respectively. In addition, a region coding for a NUDIX domain was present in ORF1. There was a 62-bp intergenic region between ORF1 and RdRp. Similarity with coat protein of mycoviruses was not found within the whole sequence. Based on phylogenetic analysis of the putative RdRp sequence, LeV grouped into a clade with dsRNA found in the basidiomycetes Phlebiopsis gigantea and Helicobasidium mompa. The clade was placed apart from the Totiviridae and Chrysoviridae families. As suggested from the genome sequence, AFM revealed that the structure of LeV was linear unencapsidated dsRNA.
conclusionsThe results suggest that LeV represents a novel family of mycoviruses, found thus far only among the basidiomycetes.
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