ArticleAnnals of laboratory medicine2012
Evaluation of DNA extraction methods and their clinical application for direct detection of causative bacteria in continuous ambulatory peritoneal dialysis culture fluids from patients with peritonitis by using broad-range PCR.
Article in Annals of laboratory medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
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- First report on mycetoma in Turkana County-North-western Kenya.PLoS neglected tropical diseases · 2023Article
- Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening inInternational journal of molecular sciences · 2023Article
- Prevalence and resistance to antibacterial agents inVeterinary world · 2023Article
- Recent advances in novel diagnostic testing for peritoneal dialysis-related peritonitis.Kidney research and clinical practice · 2022Article
- Draft Genome Sequences of 3 Strains of Apilactobacillus kunkeei Isolated from the Bee Gut Microbial Community.Microbiology resource announcements · 2021Article
- Validation of real-time PCR technique for detection of Mycobacterium bovis and Brucella abortus in bovine raw milk.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Microbial Diversity and Antimicrobial Resistance Profile in Microbiota From Soils of Conventional and Organic Farming Systems.Frontiers in microbiology · 2019Article
- The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis.Pediatric reports · 2016Article
- ISPD Peritonitis Recommendations: 2016 Update on Prevention and Treatment.Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis · 2016Review
- Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system.BioMed research international · 2014Article
- Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry.Journal of clinical microbiology · 2013Article
- Continuous Ambulatory Peritoneal Dialysis Peritonitis Guidelines - Consensus Statement of Peritoneal Dialysis Society of India - 2020.Indian journal of nephrologyReview
- Culture-Negative Peritonitis and the Latest Diagnostic Techniques.Kidney diseases (Basel, Switzerland)Review
- Use of Commercially Available Multiplex Polymerase Chain Reaction in Detection of Organism in Culture Negative Peritonitis in Peritoneal Dialysis.Indian journal of nephrologyArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aims of this study were to compare several DNA extraction methods and 16S rDNA primers and to evaluate the clinical utility of broad-range PCR in continuous ambulatory peritoneal dialysis (CAPD) culture fluids.
methodsSix type strains were used as model organisms in dilutions from 10(8) to 10(0) colony-forming units (CFU)/mL for the evaluation of 5 DNA extraction methods and 5 PCR primer pairs. Broad-range PCR was applied to 100 CAPD culture fluids, and the results were compared with conventional culture results.
resultsThere were some differences between the various DNA extraction methods and primer sets with regard to the detection limits. The InstaGene Matrix (Bio-Rad Laboratories, USA) and Exgene Clinic SV kits (GeneAll Biotechnology Co. Ltd, Korea) seem to have higher sensitivities than the others. The results of broad-range PCR were concordant with the results from culture in 97% of all cases (97/100). Two culture-positive cases that were broad-range PCR-negative were identified as Candida albicans, and 1 PCR-positive but culture-negative sample was identified as Bacillus circulans by sequencing. Two samples among 54 broad-range PCR-positive products could not be sequenced.
conclusionsThere were differences in the analytical sensitivity of various DNA extraction methods and primers for broad-range PCR. The broad-range PCR assay can be used to detect bacterial pathogens in CAPD culture fluid as a supplement to culture methods.
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