ArticleInternational journal of mass spectrometry2012
Analysis of Histone Modifications from Tryptic Peptides of Deuteroacetylated Isoforms.
Article in International journal of mass spectrometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Enrichment of histone tail methylated lysine residuesChemical science · 2024Article
- Mass Spectrometry-Based Precise Identification of Citrullinated Histones via Limited Digestion and Biotin Derivative Tag Enrichment.Analytical chemistry · 2024Article
- Application of N-Terminal Labeling Methods Provide Novel Insights into Endoproteolysis of the Prion ProteinACS chemical neuroscience · 2024Article
- Discovery of Post-Translational Modifications inMolecules (Basel, Switzerland) · 2021Article
- A 6-alkylsalicylate histone acetyltransferase inhibitor inhibits histone acetylation and pro-inflammatory gene expression in murine precision-cut lung slices.Pulmonary pharmacology & therapeutics · 2017Article
- HDAC 3-selective inhibitor RGFP966 demonstrates anti-inflammatory properties in RAW 264.7 macrophages and mouse precision-cut lung slices by attenuating NF-κB p65 transcriptional activity.Biochemical pharmacology · 2016Article
- The histone acetyltransferase p300 inhibitor C646 reduces pro-inflammatory gene expression and inhibits histone deacetylases.Biochemical pharmacology · 2016Article
- Quantitative proteomic analysis of histone modifications.Chemical reviews · 2015Review
- The bromodomain of Gcn5 regulates site specificity of lysine acetylation on histone H3.Molecular & cellular proteomics : MCP · 2014Article
- Using glycinylation, a chemical derivatization technique, for the quantitation of ubiquitinated proteins.Analytical chemistry · 2013Article
- A quantitative analysis of histone methylation and acetylation isoforms from their deuteroacetylated derivatives: application to a series of knockout mutants.Journal of mass spectrometry : JMS · 2013Article
- High energy collisions on tandem time-of-flight mass spectrometers.Journal of the American Society for Mass Spectrometry · 2013Article
- Biotinylation of lysine method identifies acetylated histone H3 lysine 79 in Saccharomyces cerevisiae as a substrate for Sir2.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The in vitro deuteroacetylation of histones obtained from biological sources has been used previously in bottom-up mass spectrometry analyses to quantitate the percent of endogenous acetylation of specific lysine sites and/or peptides. In this report, derivatization of unmodified lysine residues on histones is used in combination with high performance mass spectrometry, including combined HPLC MS/MS, to distinguish and quantitate endogenously acetylated isoforms occurring within the same tryptic peptide sequence and to extend this derivatization strategy to other post-translational modifications, specifically methylation, dimethylation and trimethylation. The in vitro deuteroacetylation of monomethylated lysine residues is observed, though dimethylated or trimethylated residues are not derivatised. Comparison of the relative intensities ascribed to the deuteroacetylated and monomethylated species with the deuteroacetylated but unmethylated analog, provides an opportunity to estimate the percent of methylation at that site. In addition to the observed fragmentation patterns, the very high mass accuracy available on the Orbitrap mass spectrometer can be used to confirm the structural isoforms, and in particular to distinguish between trimethylated and acetylated species.
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Registered trials
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