ArticleJournal of bacteriology2012
Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Adaptation-coupled CRISPRi identifies antibiotic-sensitizing genes leading to a novel plasmid selection marker.Nucleic acids research · 2026Article
- spotter: a single-nucleotide resolution stochastic simulation model of supercoiling-mediated transcription and translation in prokaryotes.Nucleic acids research · 2023Article
- Genetic components ofFrontiers in microbiology · 2023Article
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- Autogenous regulation in vivo of theRNA (New York, N.Y.) · 2020Article
- Synonymous Mutations inFrontiers in microbiology · 2020Article
- Transcriptional Responses to ppGpp and DksA.Annual review of microbiology · 2018Review
- An A/U-Rich Enhancer Region Is Required for High-Level Protein Secretion through the HlyA Type I Secretion System.Applied and environmental microbiology · 2018Article
- Suppressors of dGTP Starvation in Escherichia coli.Journal of bacteriology · 2017Article
- Operon mRNAs are organized into ORF-centric structures that predict translation efficiency.eLife · 2017Article
- Fis Is Essential for Yersinia pseudotuberculosis Virulence and Protects against Reactive Oxygen Species Produced by Phagocytic Cells during Infection.PLoS pathogens · 2016Article
- Differential Regulation of the Surface-Exposed and Secreted SslE Lipoprotein in Extraintestinal Pathogenic Escherichia coli.PloS one · 2016Article
- Chromosomal position shift of a regulatory gene alters the bacterial phenotype.Nucleic acids research · 2015Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Upstream binding of idling RNA polymerase modulates transcription initiation from a nearby promoter.The Journal of biological chemistry · 2015Article
- The metabolic potential of Escherichia coli BL21 in defined and rich medium.Microbial cell factories · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Synthesis of the Fis nucleoid protein rapidly increases in response to nutrient upshifts, and Fis is one of the most abundant DNA binding proteins in Escherichia coli under nutrient-rich growth conditions. Previous work has shown that control of Fis synthesis occurs at transcription initiation of the dusB-fis operon. We show here that while translation of the dihydrouridine synthase gene dusB is low, unusual mechanisms operate to enable robust translation of fis. At least two RNA sequence elements located within the dusB coding region are responsible for high fis translation. The most important is an AU element centered 35 nucleotides (nt) upstream of the fis AUG, which may function as a binding site for ribosomal protein S1. In addition, a 44-nt segment located upstream of the AU element and predicted to form a stem-loop secondary structure plays a prominent role in enhancing fis translation. On the other hand, mutations close to the AUG, including over a potential Shine-Dalgarno sequence, have little effect on Fis protein levels. The AU element and stem-loop regions are phylogenetically conserved within dusB-fis operons of representative enteric bacteria.
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Registered trials
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