ArticleJournal of cell science2012
Orientation-based FRET sensor for real-time imaging of cellular forces.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 120 citations in OpenAlex.
- Highly transparent elastic optical microcavities for interferometric mapping of cell mechanics.Biomedical optics express · 2025Article
- Review
- Characterization and structural basis for the brightness of mCLIFY: A novel monomeric and circularly permuted bright yellow fluorescent protein.Biophysical journal · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Myosin II tension sensors visualize force generation within the actin cytoskeleton in living cells.Journal of cell science · 2024Article
- Characterization and structural basis for the brightness of mCLIFY: a novel monomeric and circularly permuted bright yellow fluorescent protein.Research square · 2024Article
- Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cell cortex.Nature communications · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Biomaterial-based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration.Military Medical Research · 2024Review
- PIP4K2B is mechanoresponsive and controls heterochromatin-driven nuclear softening through UHRF1.Nature communications · 2023Article
- Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Small hand-designed convolutional neural networks outperform transfer learning in automated cell shape detection in confluent tissues.PloS one · 2023Article
- The spectrin cytoskeleton integrates endothelial mechanoresponses.Nature cell biology · 2022Article
- Synaptopodin stress fiber and contractomere at the epithelial junction.The Journal of cell biology · 2022Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Real-time imaging of cellular forces using optical interference.Nature communications · 2021Article
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces.Frontiers in cell and developmental biology · 2021Review
- Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology.Sensors (Basel, Switzerland) · 2020Review
- Tailoring Cellular Function: The Contribution of the Nucleus in Mechanotransduction.Frontiers in bioengineering and biotechnology · 2020Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical stress is an unmapped source of free energy in cells. Mapping the stress fields in a heterogeneous time-dependent environment like that found in cells requires probes that are specific for different proteins and respond to biologically relevant forces with minimal disturbance to the host system. To meet these goals, we have designed a genetically encoded stress sensor with minimal volume and high sensitivity and dynamic range. The new FRET-based sensor, called cpstFRET, is designed to be modulated by the angles between the donor and acceptor rather than the distance between them. Relative to other probes, it is physically smaller and exhibits a greater dynamic range and sensitivity and expresses well. For in vivo testing, we measured stress gradients in time and space in non-erythroid spectrin in several different cell types and found that spectrin is under constitutive stress in some cells but not in others. Stresses appear to be generated by both F-actin and tubulin. The probe revealed, for the first time, that spectrin undergoes time-dependent force modulation during cell migration. cpstFRET can be employed in vitro, in vivo and in situ, and when incorporated into biologically expressed extracellular polymers such as collagen, it can report multidimensional stress fields.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.