ArticleEukaryotic cell2012
The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- Trypanosome KKIP1 Dynamically Links the Inner Kinetochore to a Kinetoplastid Outer Kinetochore Complex.Frontiers in cellular and infection microbiology · 2021Article
- Dynamic colocalization of 2 simultaneously activeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Whole Exome Sequencing Identified a Novel BiallelicFrontiers in genetics · 2019Article
- Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis.PLoS neglected tropical diseases · 2017Article
- Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes.The Journal of cell biology · 2017Article
- Ribosomal RNA gene transcription in trypanosomes.Parasitology research · 2014Review
- A new strategy of RNA interference that targets heterologous sequences reveals CITFA1 as an essential component of class I transcription factor A in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis.Molecular microbiology · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
African trypanosomes are the only organisms known to use RNA polymerase I (pol I) to transcribe protein-coding genes. These genes include VSG, which is essential for immune evasion and is transcribed from an extranucleolar expression site body (ESB). Several trypanosome pol I subunits vary compared to their homologues elsewhere, and the question arises as to how these variations relate to pol I function. A clear example is the N-terminal extension found on the second-largest subunit of pol I, RPA2. Here, we identify an essential role for this region. RPA2 truncation leads to nuclear exclusion and a growth defect which phenocopies single-allele knockout. The N terminus is not a general nuclear localization signal (NLS), however, and it fails to accumulate unrelated proteins in the nucleus. An ectopic NLS is sufficient to reinstate nuclear localization of truncated RPA2, but it does not restore function. Moreover, NLS-tagged, truncated RPA2 has a different subnuclear distribution to full-length protein and is unable to build stable pol I complexes. We conclude that the RPA2 N-terminal extension does not have a role exclusive to the expression of protein-coding genes, but it is essential for all pol I functions in trypanosomes because it directs trypanosomatid-specific interactions with RPA1.
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