ArticleApplied and environmental microbiology2012
Toward homosuccinate fermentation: metabolic engineering of Corynebacterium glutamicum for anaerobic production of succinate from glucose and formate.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed, 230 citations in OpenAlex.
- Formate-driven in situ COBiotechnology for biofuels and bioproducts · 2026Article
- Perspectives for Advancing Biotechnological Succinic Acid Production.Microbial biotechnology · 2026Review
- Intestinal microbiota-host crosstalk reveals mechanisms regulating residual feed intake in egg-type ducks.BMC genomics · 2026Article
- Impact of exporter proteins and their engineering on the productivity of Corynebacterium.Applied microbiology and biotechnology · 2025Review
- Prospects of formamide as nitrogen source in biotechnological production processes.Applied microbiology and biotechnology · 2024Review
- Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.Microbial cell factories · 2024Article
- Genetic optimization of the human gut bacterium Phocaeicola vulgatus for enhanced succinate production.Applied microbiology and biotechnology · 2024Article
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
- High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects.Microbiology spectrum · 2022Article
- A manually curated compendium of expression profiles for the microbial cell factory Corynebacterium glutamicum.Scientific data · 2022Article
- Metabolic engineering of Vibrio natriegens for anaerobic succinate production.Microbial biotechnology · 2022Article
- Biosynthetic Pathway and Metabolic Engineering of Succinic Acid.Frontiers in bioengineering and biotechnology · 2022Review
- Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions.Applied microbiology and biotechnology · 2021Review
- A New Pathway for Forming Acetate and Synthesizing ATP during Fermentation in Bacteria.Applied and environmental microbiology · 2021Article
- Joint contributions of the gut microbiota and host genetics to feed efficiency in chickens.Microbiome · 2021Article
- Increasement of O-acetylhomoserine production in Escherichia coli by modification of glycerol-oxidative pathway coupled with optimization of fermentation.Biotechnology letters · 2021Article
- Genomics Characterization of an EngineeredFrontiers in bioengineering and biotechnology · 2021Article
- Application of Metabolomics in Diagnosis of Cow Mastitis: A Review.Frontiers in veterinary science · 2021Review
- Elevated, non-proliferative temperatures change the profile of fermentation products in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Multiplex Design of the Metabolic Network for Production of l-Homoserine in Escherichia coli.Applied and environmental microbiology · 2020Article
5 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Previous studies have demonstrated the capability of Corynebacterium glutamicum for anaerobic succinate production from glucose under nongrowing conditions. In this work, we have addressed two shortfalls of this process, the formation of significant amounts of by-products and the limitation of the yield by the redox balance. To eliminate acetate formation, a derivative of the type strain ATCC 13032 (strain BOL-1), which lacked all known pathways for acetate and lactate synthesis (Δcat Δpqo Δpta-ackA ΔldhA), was constructed. Chromosomal integration of the pyruvate carboxylase gene pyc(P458S) into BOL-1 resulted in strain BOL-2, which catalyzed fast succinate production from glucose with a yield of 1 mol/mol and showed only little acetate formation. In order to provide additional reducing equivalents derived from the cosubstrate formate, the fdh gene from Mycobacterium vaccae, coding for an NAD(+)-coupled formate dehydrogenase (FDH), was chromosomally integrated into BOL-2, leading to strain BOL-3. In an anaerobic batch process with strain BOL-3, a 20% higher succinate yield from glucose was obtained in the presence of formate. A temporary metabolic blockage of strain BOL-3 was prevented by plasmid-borne overexpression of the glyceraldehyde 3-phosphate dehydrogenase gene gapA. In an anaerobic fed-batch process with glucose and formate, strain BOL-3/pAN6-gap accumulated 1,134 mM succinate in 53 h with an average succinate production rate of 1.59 mmol per g cells (dry weight) (cdw) per h. The succinate yield of 1.67 mol/mol glucose is one of the highest currently described for anaerobic succinate producers and was accompanied by a very low level of by-products (0.10 mol/mol glucose).
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